College of Horticulture, Northwest Agriculture and Forest University, Yangling, 712100, Shaanxi, China.
Lingnan Guangdong Laboratory of Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518124, Guangdong, China.
Theor Appl Genet. 2020 Apr;133(4):1123-1131. doi: 10.1007/s00122-020-03536-w. Epub 2020 Jan 22.
The yellow margin (ym) gene was mapped to a 30-kb genomic region in potato and the mutation of a pectate lyase gene led to this phenotype. The practice of clonally propagating potato (Solanum tuberosum L.), which has been lasted for thousands of years, has caused the accumulation of deleterious alleles. Despite yellow margin (ym) being a common cause of a detrimental weak-vigor phenotype and reduced yield in diploid potato, the underlying gene has eluded discovery to date. In this paper, we mapped the ym gene to a 30-kb region containing four annotated genes. Among them, PGSC0003DMG402023481 encodes a pectate lyase-like gene (StPLL) with lower expression in ym plants than in the wild-type plants. PCR amplification confirmed a 4.1-kb deletion in the mutant allele of StPLL. Knockout of StPLL in diploid potato resulted in a similar phenotype with the ym plants. This study not only characterizes the ym allele but also provides the molecular tools to select and purge it from populations, while also deepening our understanding of the morphogenesis in potato.
黄色边缘（ym）基因被定位到马铃薯中的一个 30kb 基因组区域，其突变导致了这种表型。马铃薯的无性繁殖（Solanum tuberosum L.）已经持续了数千年，导致了有害等位基因的积累。尽管黄色边缘（ym）是二倍体马铃薯中一种常见的有害弱活力表型和产量降低的原因，但迄今为止，其潜在基因尚未被发现。在本文中，我们将 ym 基因定位到一个包含四个注释基因的 30kb 区域。其中，PGSC0003DMG402023481 编码一个果胶裂解酶样基因（StPLL），其在 ym 植株中的表达低于野生型植株。PCR 扩增证实突变等位基因 StPLL 存在 4.1kb 的缺失。在二倍体马铃薯中敲除 StPLL 导致与 ym 植株相似的表型。本研究不仅对 ym 等位基因进行了特征描述，还提供了从群体中选择和清除它的分子工具，同时也加深了我们对马铃薯形态发生的理解。
