Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Key Laboratory of Synthetic Biology, Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, 518120, China.
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng, 475004, China.
Theor Appl Genet. 2023 Aug 9;136(9):183. doi: 10.1007/s00122-023-04430-x.
The transcription factor StDL1 regulates dissected leaf formation in potato and the genotype frequency of recessive Stdl1/Stdl1, which results in non-dissected leaves, has increased in cultivated potatoes. Leaf morphology is a key trait of plants, influencing plant architecture, photosynthetic efficiency and yield. Potato (Solanum tuberosum L.), the third most important food crop worldwide, has a diverse leaf morphology. However, despite the recent identification of several genes regulating leaf formation in other plants, few genes involved in potato leaf development have been reported. In this study, we identified an R2R3 MYB transcription factor, Dissected Leaf 1 (StDL1), regulating dissected leaf formation in potato. A naturally occurring allele of this gene, Stdl1, confers non-dissected leaves in young seedlings. Knockout of StDL1 in a diploid potato changes the leaf morphology from dissected to non-dissected. Experiments in N. benthamiana and yeast show that StDL1 is a transcriptional activator. Notably, by calculating the genotype frequency of the Stdl1/Stdl1 in 373-potato accessions, we found that it increases significantly in cultivated potatoes. This work reveals the genetic basis of dissected leaf formation in potato and provides insights into plant leaf morphology.
转录因子 StDL1 调控马铃薯叶片的解剖结构形成，隐性 Stdl1/Stdl1 基因型的频率在栽培马铃薯中增加，导致叶片不解剖。叶片形态是植物的一个关键特征，影响植物的结构、光合作用效率和产量。马铃薯（Solanum tuberosum L.）是世界上第三重要的粮食作物，具有多样化的叶片形态。然而，尽管最近在其他植物中鉴定了几个调控叶片形成的基因，但报道的参与马铃薯叶片发育的基因很少。在这项研究中，我们鉴定了一个 R2R3 MYB 转录因子 Dissected Leaf 1（StDL1），它调控马铃薯叶片的解剖结构形成。该基因的一个自然发生的等位基因 Stdl1 在幼苗中赋予非解剖叶片。在二倍体马铃薯中敲除 StDL1 会使叶片形态从解剖结构转变为非解剖结构。在 N. benthamiana 和酵母中的实验表明，StDL1 是一个转录激活因子。值得注意的是，通过计算 373 个马铃薯品系中 Stdl1/Stdl1 的基因型频率，我们发现它在栽培马铃薯中显著增加。这项工作揭示了马铃薯叶片解剖结构形成的遗传基础，并为植物叶片形态提供了新的见解。
