Zhang Chunzhi, Yang Zhongmin, Tang Dié, Zhu Yanhui, Wang Pei, Li Dawei, Zhu Guangtao, Xiong Xingyao, Shang Yi, Li Canhui, Huang Sanwen
Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen, Guangdong 518120, China.
The AGISCAAS-YNNU Joint Academy of Potato Sciences, Yunnan Normal University, Kunming, Yunnan 650500, China.
Cell. 2021 Jul 22;184(15):3873-3883.e12. doi: 10.1016/j.cell.2021.06.006. Epub 2021 Jun 24.
Reinventing potato from a clonally propagated tetraploid into a seed-propagated diploid, hybrid potato, is an important innovation in agriculture. Due to deleterious mutations, it has remained a challenge to develop highly homozygous inbred lines, a prerequisite to breed hybrid potato. Here, we employed genome design to develop a generation of pure and fertile potato lines and thereby the uniform, vigorous Fs. The metrics we applied in genome design included the percentage of genome homozygosity and the number of deleterious mutations in the starting material, the number of segregation distortions in the S population, the haplotype information to infer the break of tight linkage between beneficial and deleterious alleles, and the genome complementarity of the parental lines. This study transforms potato breeding from a slow, non-accumulative mode into a fast-iterative one, thereby potentiating a broad spectrum of benefits to farmers and consumers.
将马铃薯从无性繁殖的四倍体转变为种子繁殖的二倍体杂交马铃薯，是农业领域的一项重要创新。由于有害突变，培育高度纯合的自交系仍然是一项挑战，而这是培育杂交马铃薯的先决条件。在此，我们采用基因组设计来培育一代纯合且可育的马铃薯品系，进而获得整齐、健壮的杂交种。我们在基因组设计中应用的指标包括起始材料的基因组纯合率和有害突变数量、S群体中的分离畸变数量、用于推断有利和有害等位基因之间紧密连锁断裂的单倍型信息，以及亲本系的基因组互补性。这项研究将马铃薯育种从缓慢、非累积的模式转变为快速迭代的模式，从而为农民和消费者带来广泛的益处。
