School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000, Thailand.
Graduate School of Science and Technology for Innovation, Yamaguchi University, Yamaguchi 753-8515, Japan.
Int J Syst Evol Microbiol. 2020 Mar;70(3):1903-1911. doi: 10.1099/ijsem.0.003993. Epub 2020 Jan 23.
Strain N24 was isolated from soil contaminated with starling's feces collected from Roi-Et province, Thailand. Cells of N24 were Gram-stain-positive rods, aerobic and non-spore-forming. N24 was positive for catalase, urease, citrate utilization, nitrate reduction and Methyl Red (MR) test but negative for oxidase, casein, gelatin liquefaction, tyrosine, Voges-Proskauer (VP) reaction and starch hydrolysis. -diaminopimelic acid, rhamnose, ribose, arabinose and galactose were detected in its whole-cell hydrolysates. The results of the 16S rRNA gene sequence analysis indicated that N24 represented a member of the genus . N24 was closely related to ATCC 13032, with 99.0 % 16S rRNA gene sequence similarity. According to results obtained using DNA-DNA hybridization approaches, N24 showed highest DNA-DNA relatedness (27.6 %) and average nucleotide identity (84.1 %) to ATCC 13032. The DNA G+C content of N24 was 51.8 mol% (genome based). The major cellular fatty acids of N24 were C, and Cω9. N24 had the nine isoprenes unit, MK-9(H) as the predominant menaquinone. The predominant polar lipids were phosphatidylglycerol, phosphatidylinositol and diphosphatidylglycerol. Mycolic acids were also present. According to the complete genome sequence data, strain N24 and ATCC 13032 are close phylogenetic neighbours, but have different genome characteristics. On the basis of the results of the genotypic and genomic studies and phenotypic characteristics including chemotaxonomy, strain N24 should be classified as representing a novel species of the genus , for which the name sp. nov. is proposed. The type strain is N24 (TBRC 5845=NBRC 113465).
菌株 N24 是从泰国罗勇府受八哥粪便污染的土壤中分离得到的。细胞呈革兰氏阳性杆状，需氧，不产芽孢。N24 触酶、脲酶、柠檬酸盐利用、硝酸盐还原和甲基红（MR）试验阳性，氧化酶、酪蛋白、明胶液化、酪氨酸、Voges-Proskauer（VP）反应和淀粉水解试验阴性。其全细胞水解物中检测到 -二氨基庚二酸、鼠李糖、核糖、阿拉伯糖和半乳糖。16S rRNA 基因序列分析结果表明，N24 代表游动放线菌属的一个成员。N24 与 ATCC 13032 最为密切，16S rRNA 基因序列相似性为 99.0%。根据 DNA-DNA 杂交方法的结果，N24 与 ATCC 13032 的 DNA-DNA 相关性最高（27.6%），平均核苷酸同一性（84.1%）。N24 的 DNA G+C 含量为 51.8mol%（基于基因组）。N24 的主要细胞脂肪酸为 C 和 Cω9。N24 有九个异戊烯单位，MK-9(H) 为主要的menaquinone。主要的极性脂是磷脂酰甘油、磷脂酰肌醇和双磷脂酰甘油。也存在分枝菌酸。根据完整的基因组序列数据，菌株 N24 和 ATCC 13032 是密切的进化近缘关系，但具有不同的基因组特征。基于基因型和基因组研究以及表型特征，包括化学分类学，菌株 N24 应被归类为游动放线菌属的一个新种，提议将其命名为. sp. nov.。模式菌株为 N24（TBRC 5845=NBRC 113465）。
