Campus Universitário de Gurupi, Universidade Federal do Tocantins, Gurupi, TO, Brazil.
Laboratório Nacional de Nanotecnologia (LNNano), Centro Nacional de Pesquisa em Energia e Materiais (CNPEM), 13083-970, Campinas, São Paulo, Brazil.
Carbohydr Res. 2020 Feb;488:107891. doi: 10.1016/j.carres.2019.107891. Epub 2019 Dec 13.
The wide use of chitosan microspheres in pharmaceutical applications, mainly in the controlled release of drugs and as a bactericidal agent, has been widely reported in the literature. However, these important biomaterial applications with multifunctionality is still scarce. In this study, epichlorohydrin-crosslinked chitosan microspheres were prepared and evaluated for Ag+ adsorption and formation of silver nanoparticles. The hybrid material obtained was used to antibacterial activity and controlled drug release. The optimal pH for adsorption of Ag+ ions in aqueous medium was pH 5-7; the best fit was the Langmuir model; the optimal time for maximum adsorption was 10 h. The highest release of the drug occured at neutral pH of the intestinal fluids and remained constant for 6 h. Silver ion microspheres demonstrated activity against E. coli and S. aureus. The concentration of ibuprofen incorporated in the chitosan microspheres was 7.9 × 10 mol L and in the microspheres with AgNPs the concentration was 1.8 × 10 mol L. The microspheres with AgNPs released more drug (77%) than the material without AgNPs.
壳聚糖微球在药物应用中的广泛使用，主要是在药物的控制释放和作为杀菌剂方面，在文献中有广泛的报道。然而，这些具有多功能性的重要生物材料的应用仍然很少。在这项研究中，制备了环氧氯丙烷交联壳聚糖微球，并对其进行了 Ag+吸附和形成银纳米粒子的评价。所得到的杂化材料用于抗菌活性和控制药物释放。在水介质中吸附 Ag+离子的最佳 pH 值为 5-7；最佳拟合是朗缪尔模型；最大吸附的最佳时间为 10 h。药物的最高释放发生在肠道液的中性 pH 值下，并保持恒定 6 h。银离子微球对大肠杆菌和金黄色葡萄球菌表现出活性。布洛芬在壳聚糖微球中的浓度为 7.9×10 mol L，在载银纳米粒子的微球中浓度为 1.8×10 mol L。载有 AgNPs 的微球释放的药物（77%）多于没有 AgNPs 的材料。
