基于测定铷负载酵母细胞中铷流出量的多烯抗生素生物测定方法。
Bioassay method for polyene antibiotics based on the measurement of rubidium efflux from rubidium-loaded yeast cells.
作者信息
Cosgrove R F, Fairbrother J E
出版信息
Antimicrob Agents Chemother. 1977 Jan;11(1):31-3. doi: 10.1128/AAC.11.1.31.
Abstract
A bioassay method for the polyene antibiotics nystatin and amphotericin B is proposed based on the measurement of the efflux of rubidium ions from a rubidium-loaded yeast culture challenged with the antibiotics. For this purpose a major proportion of the intracellular K(+) ions in a Saccharomyces cerevisiae culture has been substituted by Rb(+) ions. The rubidium leakage is measured by atomic absorption spectrophotometry, and a straight-line, dose-response correlation has been obtained for both antibiotics.
摘要
基于对用多烯抗生素攻击的、负载铷离子的酵母培养物中铷离子外流的测量，提出了一种用于多烯抗生素制霉菌素和两性霉素B的生物测定方法。为此，酿酒酵母培养物中大部分细胞内K(+)离子已被Rb(+)离子取代。通过原子吸收分光光度法测量铷泄漏，并且已获得两种抗生素的直线剂量-反应相关性。
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J Gen Microbiol. 1961 Jan;24:51-62. doi: 10.1099/00221287-24-1-51.
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