嗜热四膜虫中的表型分离：抗生素抗性标记、tsA、死亡和高度扩增的核糖体DNA位点的分离动力学
Phenotypic assortment in Tetrahymena thermophila: assortment kinetics of antibiotic-resistance markers, tsA, death, and the highly amplified rDNA locus.
作者信息
Merriam E V, Bruns P J
机构信息
Biology Department, Loyola Marymount University, Los Angeles, California 90045.
出版信息
Genetics. 1988 Oct;120(2):389-95. doi: 10.1093/genetics/120.2.389.
Abstract
Phenotypic assortment in Tetrahymena thermophila results from random distribution of alleles during amitotic division of the macronucleus. The rate of assortment is dependent on input ratio and the number of assorting units. The assortment of the antibiotic resistance markers Chx, Mpr and gal was determined and is consistent for each with the model of 45 assorting chromosomes. The gene tsA (previously ts-1) shows normal assortment, in contrast to previous reports. A mutation in the highly amplified ribosomal locus (rdnA2) assorts as if present at only 45 copies. Death of clones occurred at a rate consistent with assortment for a single gene.
摘要
嗜热四膜虫的表型分离是由于大核无丝分裂过程中 alleles 的随机分布所致。分离速率取决于输入比例和分离单位的数量。确定了抗生素抗性标记 Chx、Mpr 和 gal 的分离情况，并且每种情况都与 45 条分离染色体的模型一致。与之前的报道相反，基因 tsA（以前的 ts-1）表现出正常的分离。高度扩增的核糖体基因座（rdnA2）中的一个突变，其分离情况就好像只存在 45 个拷贝一样。克隆的死亡速率与单个基因的分离情况一致。
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