Xian Hai-Peng, Zhuo Zhong-Ling, Sun Yu-Jing, Liang Bin, Zhao Xiao-Tao
Department of Clinical Laboratory, Peking University People's Hospital, Beijing 100044, P.R. China.
Department of Clinical Laboratory, Peking University International Hospital, Beijing 102206, P.R. China.
Oncol Lett. 2018 Oct;16(4):4689-4698. doi: 10.3892/ol.2018.9199. Epub 2018 Jul 24.
Long non-coding RNAs (lncRNAs) have been demonstrated to be involved in different types of cancer, including gastric cancer. Although altered lncRNAs profiles have been observed in or around gastric cancer tissues, the diagnostic value of circulating lncRNAs in gastric cancer remains unclear. In the present study, a number of highly expressed lncRNAs, including uc001lsz, GACAT2, ABHD11-AS1, GACAT3, SUMP1P3, CHET1, TUG1, SNHG12, GAS5, PVT1, LINC00152, HOTAIR, CCAT1, H19, HULC and ZNFX1-AS1, were investigated as potential minimally invasive biomarkers for this tumor. Preliminary screening experiments revealed that ZNFX1-AS1 and HULC were differentially expressed in the plasma of gastric cancer patients and healthy control subjects. The study further examined the relative expression of ZNFX1-AS1 and HULC in the plasma of 50 matching preoperative and postoperative patients, 50 gastrointestinal stromal tumor (GIST) patients, 50 gastritis/peptic ulcer patients and 50 healthy control subjects through reverse transcription-quantitative polymerase chain reaction. The correlation of lncRNA relative expression with the general characteristics and clinicopathological factors was analyzed. It was observed that the levels of ZNFX1-AS1 and HULC in the plasma of preoperative patients were markedly higher compared with those in the plasma of GIST patients, gastritis/peptic ulcer patients and healthy control subjects, while no significant difference was detected among these three groups. Receiver operating characteristic curve analysis was also conducted to distinguish gastric cancer patients from healthy control subjects. The area under the curve was 0.85 and 0.65 for ZNFX1-AS1 and HULC, respectively. In conclusion, the results indicated that the lncRNAs ZNFX1-AS1 and HULC are promising in the clinical diagnosis of gastric cancer.
长链非编码RNA（lncRNAs）已被证明参与包括胃癌在内的不同类型癌症。尽管在胃癌组织内部或周围观察到lncRNAs谱发生改变，但循环lncRNAs在胃癌中的诊断价值仍不清楚。在本研究中，对一些高表达的lncRNAs进行了研究，包括uc001lsz、GACAT2、ABHD11-AS1、GACAT3、SUMP1P3、CHET1、TUG1、SNHG12、GAS5、PVT1、LINC00152、HOTAIR、CCAT1、H19、HULC和ZNFX1-AS1，作为该肿瘤潜在的微创生物标志物。初步筛选实验显示，ZNFX1-AS1和HULC在胃癌患者血浆和健康对照者血浆中表达存在差异。该研究通过逆转录定量聚合酶链反应进一步检测了50例术前和术后配对患者、50例胃肠道间质瘤（GIST）患者、50例胃炎/消化性溃疡患者和50例健康对照者血浆中ZNFX1-AS1和HULC的相对表达。分析了lncRNA相对表达与一般特征和临床病理因素的相关性。观察到术前患者血浆中ZNFX1-AS1和HULC水平明显高于GIST患者、胃炎/消化性溃疡患者和健康对照者血浆中的水平，而这三组之间未检测到显著差异。还进行了受试者工作特征曲线分析以区分胃癌患者和健康对照者。ZNFX1-AS1和HULC的曲线下面积分别为0.85和0.65。总之，结果表明lncRNAs ZNFX1-AS1和HULC在胃癌临床诊断中具有应用前景。
