Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin 150040, People's Republic of China.
Key Laboratory of Chinese Materia Medica, Ministry of Education of Heilongjiang University of Chinese Medicine, Harbin 150040, People's Republic of China.
Steroids. 2020 Apr;156:108583. doi: 10.1016/j.steroids.2020.108583. Epub 2020 Jan 23.
Five undescribed ergostane-type C sterols, daturmetesides A-E (1-5), were isolated from the leaves of Datura metel L. The chemical structures of these new compounds were characterized through extensive spectroscopic analysis and comparison with literatures. Among them, the absolute structures of daturmetesides A and C were unambiguously determined by X-ray crystallography. The anti-inflammatory effect of daturmetesides A-E was all tested by measuring nitric oxide production in lipopolysaccharide-activated RAW264.7 cells. Daturmetesides A, C and D moderatelylowered the NO production with IC values ranging from 17.05 ± 0.35 to 24.88 ± 0.93 μM.
从曼陀罗（Datura metel L.）的叶子中分离得到了 5 种未被描述的麦角甾烷型 C 甾体，分别命名为 daturmetesides A-E（1-5）。通过广泛的光谱分析并与文献进行比较，这些新化合物的化学结构得到了表征。其中，通过 X 射线晶体学明确确定了 daturmetesides A 和 C 的绝对结构。通过测量脂多糖激活的 RAW264.7 细胞中一氧化氮的产生来测试 daturmetesides A-E 的抗炎作用。daturmetesides A、C 和 D 适度降低了 NO 的产生，IC 值范围为 17.05 ± 0.35 至 24.88 ± 0.93 μM。
