Department of Virology, School of Public Health, Shandong University, Jinan 250012, China.
Department of Virology, School of Public Health, Shandong University, Jinan 250012, China; Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou 310051, China.
Infect Genet Evol. 2020 Jun;80:104206. doi: 10.1016/j.meegid.2020.104206. Epub 2020 Jan 23.
Virulent morbillivirus infections, including Meals Virus (MeV) and Canine Distemper Virus (CDV), caused severe immune suppression and leukopenia, while attenuated vaccine strains developed protective host immune responses. However, the detailed molecular foundations of host antiviral responses were poorly characterized. In order to better understand the interactions between attenuated vaccine and host antiviral responses, the global gene expression changes in CDV-11-infected DH82 cells, a macrophage-derived cell line from canine, were investigated by transcriptomic analysis, and portions of results were confirmed with quantitative RT-PCR. The results exhibited that 372 genes significantly up-regulated (p < .01) and 119 genes were significantly down-regulated (p < .01) in CDV-infected macrophages DH82 at 48 h p.i.. The enriched functions of the significantly up-regulated (p < .01) genes were closely associated with interferon stimulated genes (ISGs), chemokine genes and pro-inflammatory factor genes. Gene ontology and pathway analysis of differentially expressed genes (DEGs) revealed that the most significantly involved pathways in CDV-infected DH82 cells were NF-κB and TNF signaling pathway, cytokine-cytokine receptor interaction, and pathogen associated molecular patterns (PAMPs), such as Toll-like, RIG-I-like and NOD-like receptor signalings. Thus, the findings indicated that pattern recognition receptors (PRRs) possibly mediated host innate and protective antiviral immune responses in CDV-11 infected DH82 cells.
强毒麻疹病毒感染，包括麻疹病毒（MeV）和犬瘟热病毒（CDV），可导致严重的免疫抑制和白细胞减少症，而减毒疫苗株则可引发宿主保护性免疫反应。然而，宿主抗病毒反应的详细分子基础仍知之甚少。为了更好地理解减毒疫苗与宿主抗病毒反应之间的相互作用，通过转录组分析研究了犬源巨噬细胞系 DH82 细胞中 CDV-11 感染后的全基因表达变化，并用定量 RT-PCR 对部分结果进行了验证。结果表明，CDV 感染巨噬细胞 DH82 后 48 h，有 372 个基因显著上调（p <.01），119 个基因显著下调（p <.01）。显著上调基因的富集功能与干扰素刺激基因（ISGs）、趋化因子基因和促炎因子基因密切相关。差异表达基因（DEGs）的基因本体和通路分析表明，CDV 感染 DH82 细胞中最显著参与的通路是 NF-κB 和 TNF 信号通路、细胞因子-细胞因子受体相互作用以及病原体相关分子模式（PAMPs），如 Toll 样、RIG-I 样和 NOD 样受体信号通路。因此，这些发现表明模式识别受体（PRRs）可能介导了 CDV-11 感染 DH82 细胞中的宿主先天和保护性抗病毒免疫反应。
