Nascimento Kátia Cristina Silva, de Oliveira Souza Sandra Maria, Fagundes Aline, Silva Roger Magno Macedo, de Oliveira Junior Francisco Odencio Rodrigues, Xavier Samanta Cristina das Chagas, Santos Gilbert Q, Corte-Real Suzana, Barros Juliana Helena da Silva
Trypanosomatid Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
Structural Biology Laboratory, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
J Parasitol Res. 2025 Mar 7;2025:7057514. doi: 10.1155/japr/7057514. eCollection 2025.
presents aflagellar and flagellar epimastigote, trypomastigote, and spheromastigote forms in axenic cultures. Attempts to utilize trypomastigote forms of to develop in vitro and in vivo infection models have failed. To investigate the infection potential of aflagellar epimastigotes, interaction studies were performed using DH82 cells and BALB/c mouse peritoneal macrophages in Dulbecco's modified Eagle medium (DMEM)/F-12 medium supplemented with fetal bovine serum and bovine serum albumin. Light-field microscopy, scanning electron microscopy, and transmission electron microscopy were used to analyze these interactions. Regarding -macrophage interactions, the following previously unseen results were obtained: (1) the aflagellar epimastigote form of infects macrophages, and (2) epimastigotes transformed into amastigotes inside macrophages. Aflagellar epimastigotes were seen adhering to and entering macrophages and differentiating to the amastigote form; amastigotes proliferated within the parasitophorous vacuole in macrophages after 15 min. At the final time point (48 h), there were few macrophages arranged on the coverslips, but interacting with free amastigotes of , while some of the parasites changed to the flagellar epimastigote form. Considering the lack of information on and its importance in public health, this study provides new insights into the biological cycle of and parasite-host relationships.
在无细胞培养物中呈现无鞭毛体、鞭毛副鞭毛体、锥鞭毛体和球鞭毛体形式。利用锥鞭毛体形式建立体外和体内感染模型的尝试均告失败。为了研究无鞭毛副鞭毛体的感染潜力，在补充有胎牛血清和牛血清白蛋白的杜尔贝科改良 Eagle 培养基（DMEM）/F-12 培养基中，使用 DH82 细胞和 BALB/c 小鼠腹腔巨噬细胞进行了相互作用研究。利用明场显微镜、扫描电子显微镜和透射电子显微镜分析这些相互作用。关于与巨噬细胞的相互作用，获得了以下以前未见的结果：（1）的无鞭毛副鞭毛体形式感染巨噬细胞，（2）副鞭毛体在巨噬细胞内转变为无鞭毛体。可见无鞭毛副鞭毛体附着并进入巨噬细胞并分化为无鞭毛体形式；15 分钟后，无鞭毛体在巨噬细胞的寄生泡内增殖。在最后时间点（48 小时），盖玻片上排列的巨噬细胞很少，但与的游离无鞭毛体相互作用，同时一些寄生虫转变为鞭毛副鞭毛体形式。考虑到关于及其在公共卫生中的重要性的信息匮乏，本研究为的生物循环和寄生虫-宿主关系提供了新的见解。
