Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, Hubei, PR China; Key Laboratory of Molecular Biophysics, Ministry of education, Wuhan, Hubei, PR China.
Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, 1037 Luoyu Road, Wuhan 430074, Hubei, PR China; Key Laboratory of Molecular Biophysics, Ministry of education, Wuhan, Hubei, PR China.
Sci Total Environ. 2020 Apr 20;714:136400. doi: 10.1016/j.scitotenv.2019.136400. Epub 2020 Jan 11.
Apparent solubility and bioavailability of hydrophobic compounds are the major problems in the bioremediation process, which could be overcome by the bacteria capable of biosurfactant production and concurrent hydrocarbon degradation. In this work, we constructed an artificial bacterial consortium containing Lysinibacillus, Paenibacillus, Gordonia and Cupriavidus spp. from glyceryl tributyrate enriched bacteria collected from the non-contaminated site. The consortium was capable of using common raw materials (olive oil, paraffin oil, and glycerol) and polyaromatic hydrocarbons pollutants (naphthalene and anthracene) as the sole carbon source with simultaneous biosurfactant production. Two new lipopeptide isoforms, containing heptapeptide and lipid moieties, were structurally elucidated by LC-MS/MS, FTIR, NMR and molecular networking analysis. Our findings indicate that hydrocarbons degradation and biosurfactant production is an intrinsic property of non-contaminated soil community. Interestingly, we observed the hyper chemotactic activity of Lysinibacillus strains towards glyceryl tributyrate, which has not been reported before. The study may deepen our understanding of microbial strains and consortium with the potential to be used for bioremediation of hydrocarbons contaminated environments.
疏水性化合物的表观溶解度和生物利用度是生物修复过程中的主要问题，这可以通过能够同时产生生物表面活性剂和降解烃类的细菌来克服。在这项工作中，我们从非污染地点富集的甘油三丁酸酯中分离出的产细菌中构建了一个含有Lysinibacillus、Paenibacillus、Gordonia 和 Cupriavidus spp 的人工细菌联合体。该联合体能够使用常见的原料（橄榄油、石蜡油和甘油）和多环芳烃污染物（萘和蒽）作为唯一的碳源，并同时产生生物表面活性剂。通过 LC-MS/MS、FTIR、NMR 和分子网络分析，对两种新型脂肽同型物进行了结构阐明，它们含有七肽和脂质部分。我们的研究结果表明，烃类降解和生物表面活性剂的产生是未受污染土壤群落的固有特性。有趣的是，我们观察到Lysinibacillus 菌株对甘油三丁酸酯的超趋化活性，这以前尚未报道过。这项研究可能加深我们对具有用于烃类污染环境生物修复潜力的微生物菌株和联合体的理解。
