Department of Life Sciences, University of Modena and Reggio Emilia, Reggio Emilia, Italy.
Department of Agrarian and Forestry Science, Catholic University of Maule, Talca, Chile.
Biotechnol Lett. 2020 May;42(5):807-818. doi: 10.1007/s10529-020-02811-6. Epub 2020 Jan 25.
The objective of this study was to evaluate the ability of a new Komagataeibacter xylinus strain in producing bacterial cellulose from glucose, mannitol and glycerol, and to assess the genome sequencing with special focus on bacterial cellulose related genes.
Bacterial cellulose production during 9 days of cultivation was tested in glucose, mannitol and glycerol, respectively. Differences in the bacterial cellulose kinetic formation was observed, with a final yield of 9.47 g/L in mannitol, 8.30 g/L in glycerol and 7.57 g/L in glucose, respectively. The draft genome sequencing of K1G4 was produced, revealing a genome of 3.09 Mbp. Two structurally completed cellulose synthase operons and a third copy of the catalytic subunit of cellulose synthase were found. By using phylogenetic analysis, on the entire rRNA operon sequence, K1G4 was found to be closely related to Komagataeibacter xylinus LMG 1515 and K. xylinus K2G30.
The different yields of bacterial cellulose produced on glucose, mannitol and glycerol can be correlated with the third copy of bcsAB operon harboured by K1G4, making it a versatile strain for industrial applications.
本研究旨在评估一株新型木醋杆菌（Komagataeibacter xylinus）利用葡萄糖、甘露醇和甘油生产细菌纤维素的能力，并对其基因组测序进行评估，特别关注与细菌纤维素相关的基因。
分别在葡萄糖、甘露醇和甘油中培养 9 天，测试细菌纤维素的生产情况。观察到细菌纤维素形成动力学的差异，最终在甘露醇中的产量为 9.47 g/L，在甘油中为 8.30 g/L，在葡萄糖中为 7.57 g/L。完成了 K1G4 的基因组测序，揭示了一个 3.09 Mbp 的基因组。发现了两个结构完整的纤维素合酶操纵子和纤维素合酶催化亚基的第三个拷贝。通过对整个 rRNA 操纵子序列进行系统发育分析，发现 K1G4 与木醋杆菌 LMG 1515 和 K. xylinus K2G30 密切相关。
在葡萄糖、甘露醇和甘油上产生的不同产量的细菌纤维素可以与 K1G4 中携带的 bcsAB 操纵子的第三个拷贝相关，这使其成为工业应用的多功能菌株。
