Xu Nan, Qiao Lixing, Yin Liping, Li Han
Department of Pediatrics, Zhongda Hospital, School of Medicine, Southeast University, Nanjing, China.
J BUON. 2019 Nov-Dec;24(6):2273-2279.
The vital role of long noncoding RNAs (lncRNAs) in tumor progression have been identified in numerous studies. In this research, lncRNA ROR1-AS1 was explored to verify its function during the development of lung adenocarcinoma (LAC).
Real-time quantitative polymerase chain reaction (RT-qPCR) was utilized to measure ROR1-AS1 expression of LAC tissues. Function assays including wound healing assay and transwell assay were conducted to detect the effect of knockdown of ROR1-AS1 on the metastasis of LAC, and luciferase assays and RNA immunoprecipitation assay (RIP) were also performed to explore the underlying mechanism.
ROR1-AS1 expression level was significantly higher in LAC samples compared with that in adjacent tissues, which was associated with patients' prognosis. Knockdown of ROR1-AS1 inhibited cell migration and cell invasion of LAC cells via suppressing epithelial-mesenchymal transition (EMT) process. Furthermore, it was discovered that ROR1-AS1 acted as a competing endogenous RNA via sponging miR-375 in LAC.
These results suggested that ROR1-AS1 could act as a sponge for miR-375 and promo//e cell migration and invasion through suppressing the process of EMT in LAC, which may offer a potential therapeutic target in LAC.
众多研究已证实长链非编码RNA（lncRNA）在肿瘤进展中发挥着重要作用。本研究中，对lncRNA ROR1-AS1进行了探索，以验证其在肺腺癌（LAC）发生发展过程中的功能。
采用实时定量聚合酶链反应（RT-qPCR）检测LAC组织中ROR1-AS1的表达。进行了包括伤口愈合试验和Transwell试验在内的功能分析，以检测敲低ROR1-AS1对LAC转移的影响，还进行了荧光素酶试验和RNA免疫沉淀试验（RIP）以探究潜在机制。
与癌旁组织相比，LAC样本中ROR1-AS1表达水平显著更高，且与患者预后相关。敲低ROR1-AS1通过抑制上皮-间质转化（EMT）过程抑制LAC细胞的迁移和侵袭。此外，研究发现ROR1-AS1在LAC中通过海绵吸附miR-375作为竞争性内源性RNA发挥作用。
这些结果表明，ROR1-AS1可作为miR-375的海绵，通过抑制LAC中的EMT过程促进细胞迁移和侵袭，这可能为LAC提供一个潜在的治疗靶点。
