Hu Haifeng, Wang Ying, Zhang Tianyu, Zhang Chunyu, Liu Ying, Li Guoan, Zhou Dandan, Lu Shengnan
Department of MRI, the second Affiliated Hospital of Qiqihar Medical University, Qiqihar 161006, P.R. China.
J BUON. 2019 Nov-Dec;24(6):2377-2384.
This study was designed to investigate the relationship between the abnormal expression of LncRNA GACAT3 and the prognosis of breast cancer patients, preoperative magnetic resonance imaging (MRI) parameters and the molecular downstream mechanism.
Quantitative fluorescence PCR was used to detect the expression of LCCRNA GACAT3 in 20 breast cancer tissues and adjacent tissues. Patients were divided into low expression group and high expression group according to the level of expression, and the differences and overall survival of MRI diffusion-weighted imaging parameters were analyzed. The expression of LCCRNA GACAT3 was interfered by MCV-7 cells transfected by recombinant adenovirus, and the proliferation and apoptosis of MCF-7 were detected by BrdU method and TUNEL method, respectively.
The expression of LncRNA GACAT3 was increased in breast cancer tissues and cell lines compared to paracancer tissues and normal cells. Compared with the low expression group, patients with high expression had poorer MRI diffusionweighted imaging and lower overall survival. Down-regulation of LncRNA GACAT3 increased the expression of miR-497, and miR-497 mimics reduced the luciferase of LncRNA GACAT3. Increased LrcRNA GACAT3 in breast cancer cells could downregulate the expression of miR-497, down-regulate Capsase 9 and up-regulate Bcl-2 to promote proliferation and anti-apoptosis of breast cancer cells.
LncRNA GAC AT3 is associated with poor prognosis of breast cancer, preoperative MRI perfusion-related diffusion (D) reduction, and elevated perfusion fraction (f). After targeting CIR-497, LncRNA GACAT3 promotes the progression of breast cancer by down- regulating Caspase 9 and up-regulating Bcl-2.
本研究旨在探讨长链非编码RNA（LncRNA）GACAT3的异常表达与乳腺癌患者预后、术前磁共振成像（MRI）参数及分子下游机制之间的关系。
采用定量荧光PCR检测20例乳腺癌组织及癌旁组织中LCCRNA GACAT3的表达。根据表达水平将患者分为低表达组和高表达组，分析MRI扩散加权成像参数的差异及总生存期。通过重组腺病毒转染的MCV-7细胞干扰LCCRNA GACAT3的表达，分别采用BrdU法和TUNEL法检测MCF-7的增殖和凋亡情况。
与癌旁组织和正常细胞相比，LncRNA GACAT3在乳腺癌组织和细胞系中的表达升高。高表达患者的MRI扩散加权成像较低表达组差，总生存期也较低。LncRNA GACAT3的下调增加了miR-497的表达，miR-497模拟物降低了LncRNA GACAT3的荧光素酶活性。乳腺癌细胞中LrcRNA GACAT3的增加可下调miR-497的表达，下调Caspase 9并上调Bcl-2，从而促进乳腺癌细胞的增殖和抗凋亡。
LncRNA GAC AT3与乳腺癌预后不良、术前MRI灌注相关扩散（D）降低及灌注分数（f）升高有关。靶向CIR-497后，LncRNA GACAT3通过下调Caspase 9和上调Bcl-2促进乳腺癌进展。
