Laboratory of Human Pathology Biomedicine and Environment, Faculty of Medicine and Pharmacy of Fez (FMPF), Sidi Mohammed Ben Abdellah University (USMBA), Fez, Morocco.
Faculty of Sciences and Techniques of Fez (FSTF), USMBA, Fez, Morocco.
Lett Appl Microbiol. 2020 Apr;70(4):241-251. doi: 10.1111/lam.13278. Epub 2020 Feb 11.
Food poisoning risk related to the consumption of contaminated food with known foodborne pathogens or antibiotic-resistant bacteria is currently a serious threat for public health. Thus, pathogenic methicillin-resistant Staphylococcus strains are considered as one of the major cause of foodborne diseases in hospitals. The present study aims to determine the prevalence and the antibiotic resistance patterns of Staphylococcus in various types of hospital food samples, work surfaces and its carriage by food handlers. A total of 608 collected samples including 300 food samples, 238 food contact surfaces and 70 nasal and hand samples were tested. The identified Staphylococcus and their antibiotic resistance patterns were analysed using the agar disk-diffusion and PCR method was used for mecA resistance gene amplification. The prevalence of S. aureus and the coagulase-negative staphylococci were 17·33 and 23·33%, respectively. The antibiotic resistance reached 100% towards oxacillin and Penicillin G for both S. aureus and CoNs. The mecA gene was detected in 5·71% (4/70) and 7·69% (4/52) of S. aureus and CoNs strains, respectively. The outcome of this study enlightens isolation of MRSA strains and resistant CoNs from food, food contact surfaces and food handlers. The presence of this resistant species in this critical setting, where products were intended to vulnerable and immunocompromised patients, represents a serious threat to this community. It can be a source of nosocomial infection and more precautions must be taken to prevent staphylococci food contamination mainly in hospitals. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first study describing the antibiotic resistance patterns of Staphylococcus aureus and coagulase-negative Staphylococcus isolated from hospital food, food contact surfaces and food handlers samples in a Moroccan hospital kitchen. High levels of multi-resistance were reported. The alarming outcome of this study emphasizes the crucial need of implementing an approach to fight multidrug-resistant staphylococci mainly in healthcare settings, where the community have already compromised health issues.
食源性疾病风险与食用受已知食源性病原体或抗生素耐药细菌污染的食物有关，目前对公众健康构成严重威胁。因此，耐甲氧西林金黄色葡萄球菌菌株被认为是医院食源性疾病的主要原因之一。本研究旨在确定各种医院食品样本、工作表面及其食品处理人员携带的葡萄球菌的流行率和抗生素耐药模式。共采集了 608 个样本，包括 300 个食品样本、238 个食品接触面和 70 个鼻和手部样本。使用琼脂平板扩散法和聚合酶链反应（PCR）方法检测鉴定的葡萄球菌及其抗生素耐药模式，并用 PCR 方法检测 mecA 耐药基因扩增。金黄色葡萄球菌和凝固酶阴性葡萄球菌的检出率分别为 17.33%和 23.33%。金黄色葡萄球菌和凝固酶阴性葡萄球菌对苯唑西林和青霉素 G 的耐药率均为 100%。mecA 基因在 5.71%（4/70）和 7.69%（4/52）的金黄色葡萄球菌和凝固酶阴性葡萄球菌菌株中均有检出。本研究结果表明，从食品、食品接触面和食品处理人员中分离出耐甲氧西林金黄色葡萄球菌（MRSA）菌株和耐药凝固酶阴性葡萄球菌。在这种关键环境中存在这种耐药物种，而这些产品是为脆弱和免疫功能低下的患者准备的，这对该社区构成了严重威胁。它可能是医院感染的来源，必须采取更多预防措施来防止主要在医院发生的葡萄球菌食品污染。研究的意义和影响：这是首次描述从摩洛哥医院厨房的医院食品、食品接触面和食品处理人员样本中分离出的金黄色葡萄球菌和凝固酶阴性葡萄球菌的抗生素耐药模式。报告了高水平的多药耐药性。本研究令人震惊的结果强调了在已经存在健康问题的医疗保健环境中实施控制多药耐药葡萄球菌措施的迫切需要。
