Silva Vanessa, Vieira-Pinto Madalena, Saraiva Cândido, Manageiro Vera, Reis Lígia, Ferreira Eugénia, Caniça Manuela, Capelo José L, Igrejas Gilberto, Poeta Patrícia
Department of Veterinary Sciences, University of Trás-os-Montes and Alto Douro (UTAD), 5000-801 Vila Real, Portugal.
Department of Genetics and Biotechnology, Functional Genomics and Proteomics' Unit, University of Trás-os-Montes and Alto Douro, 5000-801 Vila Real, Portugal.
Animals (Basel). 2021 Jul 8;11(7):2038. doi: 10.3390/ani11072038.
Livestock-associated MRSA (LA-MRSA) is a zoonotic pathogen that has been reported in several animals, and it is often associated with clonal complex (CC) 398. We aimed to isolate MRSA from quails and to characterize their antimicrobial resistance and genetic lineages. One hundred swab samples were recovered from quails at the slaughterhouse. The swabs were inoculated onto CHROMagar™ MRSA agar plates for MRSA isolation. The presence of antimicrobial-resistant genes and virulence factors was investigated by PCR. All strains were typed by MLST, SCC-, - and -typing. From the 100 samples, 29 MRSA were isolated. All strains were resistant to penicillin, cefoxitin, ciprofloxacin, erythromycin and clindamycin and carried the Z, A, B and C genes. All strains, except one, showed resistance to tetracycline and harbored the M, K and L genes in different combinations. Twenty strains belonged to ST398 and SCC type V, and nine strains belonged to the new ST6831. Twenty-eight out of twenty-nine strains were ascribed to t011 and one to t108. As far as we know, this is the first report of MRSA from quails slaughtered for human consumption. Most strains belonged to ST398-t011, which is the most common LA-MRSA clone found in livestock in Europe.
家畜相关的耐甲氧西林金黄色葡萄球菌（LA-MRSA）是一种人畜共患病原体，已在多种动物中被报道，且常与克隆复合体（CC）398相关。我们旨在从鹌鹑中分离出耐甲氧西林金黄色葡萄球菌，并对其抗菌耐药性和基因谱系进行表征。从屠宰场的鹌鹑中采集了100份拭子样本。将拭子接种到CHROMagar™ MRSA琼脂平板上以分离耐甲氧西林金黄色葡萄球菌。通过聚合酶链反应（PCR）研究抗菌耐药基因和毒力因子的存在情况。所有菌株通过多位点序列分型（MLST）、葡萄球菌染色体盒式Mec（SCC）分型和spa分型进行分型。从100个样本中分离出29株耐甲氧西林金黄色葡萄球菌。所有菌株对青霉素、头孢西丁、环丙沙星、红霉素和克林霉素耐药，并携带Z基因群、A基因群、B基因群和C基因群。除一株外，所有菌株对四环素耐药，并以不同组合携带M基因群、K基因群和L基因群。20株属于ST398和V型SCC，9株属于新的ST6831。29株菌株中有28株归为t011型，1株归为t108型。据我们所知，这是首次关于供人类食用的屠宰鹌鹑中耐甲氧西林金黄色葡萄球菌的报告。大多数菌株属于ST398-t011，这是在欧洲家畜中发现的最常见的LA-MRSA克隆。
