一种超灵敏且可见的点亮探针，用于在水样、血清中对噻吩酚进行成像，并可视化活细胞中噻吩酚诱导的氧化应激过程。
An ultrasensitive and visible lighting-up probe for imaging thiophenols in water samples, in serum and visualizing thiophenols-induced oxidative stress process in live cells.
机构信息
Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province and State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou, 730000, PR China.
State Key Laboratory of Veterinary Etiological Biology and Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Xujiaping 1, Lanzhou, Gansu Province, 730046, PR China.
出版信息
Talanta. 2020 Apr 1;210:120622. doi: 10.1016/j.talanta.2019.120622. Epub 2019 Dec 9.
Thiophenols, a class of significant industrial materials, are extremely toxic in environmental as well live cells. However, the process of live cells responding to thiophenols is not well understood. Herein, an innovative "OFF-ON" probe FY for thiophenols selectively in 100% aqueous solution was reported. It featured rapid response (150 s), prominent sensitivity (detection limit: 5 nM), and large Stokes shift (104 nm), which assured specific detection of thiophenols in A375 cells, HeLa cells and environment. Especially, it proved that thiophenols in live cells can be eliminated by endogenous reactive oxygen species (ROS), indicating that thiophenols may result in cellular oxidative stress. As well, it was resoundingly put into recognizing of thiophenols quantitatively in actual water samples and in serum.
噻吩酚类物质是一类重要的工业材料，对环境和活细胞都具有极高的毒性。然而，活细胞对噻吩酚类物质的反应过程尚不清楚。本文报道了一种新型的噻吩酚类物质选择性“OFF-ON”探针 FY，它可在 100%水溶液中快速响应（150 s）、高灵敏度（检测限：5 nM）和大斯托克斯位移（104 nm），可特异性检测 A375 细胞、HeLa 细胞和环境中的噻吩酚类物质。特别地，它证明了活细胞中的噻吩酚类物质可以被内源性活性氧（ROS）清除，表明噻吩酚类物质可能导致细胞氧化应激。此外，它还成功地用于实际水样和血清中噻吩酚类物质的定量识别。
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