用于苯硫酚的具有显著斯托克斯位移的简单近红外发射激发态质子转移荧光探针及其应用

Simple NIR-Emitting ESIPT Fluorescent Probe for Thiophenol with a Remarkable Stokes Shift and Its Application.

作者信息

Li Zhiying, Wu Yang, Shen Youming, Gu Biao

机构信息

Hunan Province Engineering Research Center of Electroplating Wastewater Reuse Technology, Hunan Provincial Key Laboratory of Water Treatment Functional Materials, Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic ZoneCollege of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, P. R. China.

Key Laboratory of Functional Organometallic Materials of College of Hunan Province, College of Chemistry and Materials Science, Hengyang Normal University, Hengyang 421008, P. R. China.

出版信息

ACS Omega. 2020 May 4;5(19):10808-10814. doi: 10.1021/acsomega.0c00389. eCollection 2020 May 19.

Thiophenol as a highly toxic compound can harm the environment and living organisms and thus demands effective detection. In this work, we presented a near-infrared fluorescent probe () for detecting thiophenol according to the ESIPT mechanism using 2,4-dinitrophenyl group as a recognition unit. This probe displayed specificity toward thiophenol over other related analytes. Meanwhile, there was good linearity between the relative fluorescence intensity of and the concentration of thiophenol in the range of 0-80 μM. This probe also showed a low detection limit of 3.8 × 10 and a marked Stokes shift (192 nm). Further, this probe could be used for monitoring thiophenol in environmental water samples and imaging thiophenol in living cells, which indicated that this probe had a real application in the environment and living organisms.

苯硫酚作为一种剧毒化合物会对环境和生物造成危害，因此需要进行有效检测。在这项工作中，我们提出了一种近红外荧光探针（），它以2,4-二硝基苯基作为识别单元，根据激发态分子内质子转移（ESIPT）机制来检测苯硫酚。该探针对苯硫酚相对于其他相关分析物具有特异性。同时，在0 - 80 μM范围内，其相对荧光强度与苯硫酚浓度之间具有良好的线性关系。该探针还显示出3.8×10的低检测限和显著的斯托克斯位移（192 nm）。此外，该探针可用于监测环境水样中的苯硫酚以及对活细胞中的苯硫酚进行成像，这表明该探针在环境和生物体内具有实际应用价值。