苜蓿根多糖的结构特征、硒化修饰、抗氧化和抗肿瘤作用。
Structural features, selenization modification, antioxidant and anti-tumor effects of polysaccharides from alfalfa roots.
机构信息
College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, PR China; Institute of Functional Molecules, Shenyang University of Chemical Technology, Shenyang 110142, PR China.
College of Pharmaceutical and Biological Engineering, Shenyang University of Chemical Technology, Shenyang 110142, PR China.
出版信息
Int J Biol Macromol. 2020 Apr 15;149:207-214. doi: 10.1016/j.ijbiomac.2020.01.239. Epub 2020 Jan 24.
Hot water extraction and chromatographic purification methods were used to extract and purify two polysaccharides (RAPS-1 and RAPS-2) from the roots of alfalfa. Subsequently, RAPS-2 was modified using the HNO/NaSeO method to obtain Se-RAPS-2. The structural features, antioxidant and in vitro anti-tumor activities of the three polysaccharides were evaluated. The structural analysis revealed that RAPS-1 (Mw = 10.0 kDa) was composed of rhamnose, xylose, arabinose, galacturonic acid, mannose and glucose, whereas RAPS-2 (Mw = 15.8 kDa) consisted of rhamnose, xylose, galacturonic acid, mannose, glucose and galactose. RAPS-1 contained 1 → 2, 1 → 4, 1 → 3, and 1 → 6 or 1 → glycosidic bonds; however, while RAPS-2 lacked 1 → 4 glycosidic linkages. The molecular weight of Se-RAPS-2 was 11.0 kDa less than that of RAPS-2. The results of activities demonstrated that Se-RAPS-2 displayed superior antioxidant activity and inhibitory effect in HepG2 cells than RAPS-1 and RAPS-2.
采用热水提取和色谱纯化方法从紫花苜蓿根中提取和纯化两种多糖（RAPS-1 和 RAPS-2）。随后，采用 HNO/NaSeO 法对 RAPS-2 进行修饰，得到 Se-RAPS-2。评价了三种多糖的结构特征、抗氧化和体外抗肿瘤活性。结构分析表明，RAPS-1（Mw=10.0 kDa）由鼠李糖、木糖、阿拉伯糖、半乳糖醛酸、甘露糖和葡萄糖组成，而 RAPS-2（Mw=15.8 kDa）由鼠李糖、木糖、半乳糖醛酸、甘露糖、葡萄糖和半乳糖组成。RAPS-1 含有 1→2、1→4、1→3 和 1→6 或 1→糖苷键；然而，RAPS-2 缺乏 1→4 糖苷键。Se-RAPS-2 的分子量比 RAPS-2 小 11.0 kDa。活性结果表明，与 RAPS-1 和 RAPS-2 相比，Se-RAPS-2 在 HepG2 细胞中具有更好的抗氧化活性和抑制作用。
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