Department of Nutrition, Szent István University, H-2103 Gödöllő, Hungary.
Department of Environmental Safety and Ecotoxicology, Szent István University, H-2103 Gödöllő, Hungary.
Toxins (Basel). 2020 Jan 26;12(2):84. doi: 10.3390/toxins12020084.
Effects of aflatoxin B1 (AFB1) on lipid peroxidation and glutathione system were investigated in chicken liver. In a three-week feeding trial, different doses (<1.0 μg/kg (control diet), 17.0 µg (diet A1), 92.0 µg (diet A2), and 182.0 µg (diet A3) AFB1 kg/feed) were used. Markers of lipid peroxidation, conjugated dienes and trienes showed higher values in A3, while amounts of thiobarbituric acid reactive substances were increased in the A1 group at day 21. Glutathione content was lower at day 14 in Group A2. Glutathione peroxidase 4 activity was increased at days 7 and 21 in the A3 group but reduced in the A2 and A3 groups at day 14. The gene was downregulated at day 7 in the A2 group, but overregulated at days 14 and 21, and at day 14 in the A3 group. was downregulated at day 14 in the A1 group but overregulated at day 21 in A1 and A2 groups. was downregulated at days 7 and 21 in all treatment groups, but on day 14, induction was observed in the A3 group. The results indicated that AFB1 did not induce dose- or time-dependent effects on the glutathione redox system and its encoding genes at the dose range used, which means that oxidative stress is not the primary effect of AFB1 toxicity.
本研究旨在探讨黄曲霉毒素 B1（AFB1）对鸡肝脏脂质过氧化和谷胱甘肽系统的影响。在为期三周的喂养试验中，使用了不同剂量（<1.0 μg/kg（对照饮食）、17.0 μg（饮食 A1）、92.0 μg（饮食 A2）和 182.0 μg（饮食 A3）AFB1/kg 饲料）。在第 21 天，A3 组的共轭二烯和三烯标志物显示出更高的值，而 A1 组的硫代巴比妥酸反应物质含量增加。在第 14 天，A2 组的谷胱甘肽含量较低。在第 7 和 21 天，A3 组的谷胱甘肽过氧化物酶 4 活性增加，但在第 14 天，A2 和 A3 组的活性降低。在第 7 天，A2 组的 基因下调，但在第 14 和 21 天以及第 14 天，A3 组的基因上调。在第 14 天，A1 组的 基因下调，但在 A1 和 A2 组中，该基因在第 21 天上调。在所有处理组中，在第 7 和 21 天， 基因下调，但在第 14 天，A3 组观察到诱导。结果表明，在使用的剂量范围内，AFB1 对谷胱甘肽氧化还原系统及其编码基因没有诱导剂量或时间依赖性作用，这意味着氧化应激不是 AFB1 毒性的主要作用。
