1Department of Nutrition, Szent István University, Páter K. u. 1, H-2103 Gödöllő, Hungary.
3MTA-KE-SZIE Mycotoxins in the Food Chain Research Group, Kaposvár University, Kaposvár, Hungary.
Acta Vet Hung. 2021 Mar 24;69(1):23-30. doi: 10.1556/004.2021.00002.
The purpose of the present study was to use oxidative stress markers for investigating the effect of zeolite (315 mg/kg of complete feed) in the case of aflatoxin B1 contamination (92 μg/kg complete feed). In a 21-day feeding trial with broiler chickens, oxidative stress parameters such as conjugated dienes, conjugated trienes, malondialdehyde, reduced glutathione content and glutathione peroxidase activity were not changed significantly by supplementation with this mycotoxin absorbent. The relative gene expression of transcription factors KEAP1 and NRF2 was not modified by the absorbent either. Still, the expression of GSS, GSR and GPX4 genes increased significantly due to the aluminosilicate supplementation. The results suggest that zeolite reduced lipid peroxidation in the blood plasma but not in the red blood cell haemolysate or the kidney. The relative expression of the genes encoding the glutathione redox system also changed as a result of zeolite supplementation, but these changes were not found at the protein level.
本研究旨在利用氧化应激标志物来研究沸石（占全价饲料 315mg/kg）对黄曲霉毒素 B1 污染（占全价饲料 92μg/kg）的影响。在一项为期 21 天的肉鸡饲养试验中，补充这种霉菌毒素吸附剂并没有显著改变共轭二烯、共轭三烯、丙二醛、还原型谷胱甘肽含量和谷胱甘肽过氧化物酶活性等氧化应激参数。吸附剂也没有改变转录因子 KEAP1 和 NRF2 的相对基因表达。然而，由于添加了铝硅酸盐，GSS、GSR 和 GPX4 基因的表达显著增加。研究结果表明，沸石降低了血浆中的脂质过氧化，但没有降低红细胞溶血物或肾脏中的脂质过氧化。谷胱甘肽氧化还原系统编码基因的相对表达也因沸石的添加而发生变化，但这些变化在蛋白质水平上没有发现。
