and activity of chelerythrine against and underlying mechanisms.

To evaluate whether chelerythrine (CHT) exhibited antifungal activity against and and to explore the underlying mechanisms. Broth microdilution assay and model were used to evaluate the antifungal effect and , respectively. Mechanism studies were investigated by morphogenesis observation, Fluo-3/AM, DCFH-DA and rhodamine6G assay, respectively. CHT exhibited antifungal activity against and preformed biofilms with minimum inhibitory concentrations ranged from 2 to 16 μg/ml. Besides, CHT protected larvae infected by . Mechanisms studies revealed that CHT inhibited hyphal growth, increased intracellular calcium concentration, induced accumulation of reactive oxygen species and inhibited drug transporter activity. CHT exhibited antifungal activity against .