Jeng T W, Talmon Y, Chiu W
University Department of Biochemistry, University of Arizona, Tucson 85721.
J Electron Microsc Tech. 1988 Apr;8(4):343-8. doi: 10.1002/jemt.1060080402.
A guillotine-type quick freezing device and a bio-hazard containment box have been designed, constructed, and used to prepare vitrified-hydrated specimens of viruses in their native environment. Special design considerations include the preservation of the specimen in its natural state in vitrified ice, prevention of virus aerosols escape, and control of the potentially explosive air-coolant vapor mixtures.
一种断头台式快速冷冻装置和一个生物危害防护箱已被设计、制造并用于在病毒的天然环境中制备玻璃化水合病毒标本。特殊的设计考虑因素包括将标本保存在玻璃化冰中的自然状态、防止病毒气溶胶逸出以及控制潜在的爆炸性空气冷却剂蒸汽混合物。