Talmon Y, Prasad B V, Clerx J P, Wang G J, Chiu W, Hewlett M J
J Virol. 1987 Jul;61(7):2319-21. doi: 10.1128/JVI.61.7.2319-2321.1987.
La Crosse (LAC) virions were cryopreserved by rapid freezing in a thin layer of vitreous ice. The vitrified-hydrated LAC virions were subsequently imaged at -170 degrees C in a transmission electron microscope equipped with a low-temperature specimen holder. This cryoelectron microscopic technique eliminates the artifacts frequently associated with negative staining. Images of vitrified-hydrated LAC virions clearly revealed surface spikes as well as bilayer structure. Size measurements of the vitrified-hydrated LAC virions showed heterogeneity, with diameters ranging from 75 to 115 nm. Regardless of the particle size, the spike was about 10 nm long, and the bilayer was about 4 nm thick. The spikes are interpreted to be one or both of the glycoproteins, and the bilayer is interpreted to be the membrane envelope of the virus. In contrast to the pleomorphic appearance of the negatively stained LAC virions, the vitrified-hydrated LAC virions showed uniform spherical shapes regardless of their sizes.
拉克罗斯（LAC）病毒粒子通过在薄的玻璃态冰层中快速冷冻进行低温保存。随后，在配备低温样品架的透射电子显微镜中于-170℃对玻璃态水合LAC病毒粒子进行成像。这种低温电子显微镜技术消除了经常与负染色相关的伪像。玻璃态水合LAC病毒粒子的图像清楚地显示出表面刺突以及双层结构。对玻璃态水合LAC病毒粒子的尺寸测量显示出异质性，直径范围为75至115纳米。无论颗粒大小如何，刺突约10纳米长，双层约4纳米厚。刺突被解释为一种或两种糖蛋白，双层被解释为病毒的膜包膜。与负染色的LAC病毒粒子的多形外观相反，玻璃态水合LAC病毒粒子无论大小均呈现均匀的球形。
