通过修改枯草芽孢杆菌中相关的代谢途径来提高尿苷的产量。

Improve uridine production by modifying related metabolic pathways in Bacillus subtilis.

机构信息

Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People's Republic of China.

Key Laboratory of Systems Bioengineering (Tianjin University), Ministry of Education, Tianjin University, Tianjin, 300072, People's Republic of China.

出版信息

Biotechnol Lett. 2020 Apr;42(4):551-555. doi: 10.1007/s10529-020-02820-5. Epub 2020 Jan 28.

DOI:10.1007/s10529-020-02820-5

PMID:31993847

Abstract

OBJECTIVES

The metabolic pathway related to uridine production was modified in Bacillus subtilis in order to increase the production of uridine.

RESULTS

Decreasing the relative transcriptional level of pur operon in Bacillus subtilis TD300 to 80%, and the production of the derived strain TD312 was increased to 11.81 g uridine/l and the yield was increased to 270 mg uridine/g glucose. The expression of pucR gene in situ by P resulting in a 194.01-fold increase in the relative transcriptional level of pucR gene and 349.71-fold increase in the relative transcriptional level of ure operon, respectively. Furthermore, the production of TD314 reached 13.06 g uridine/l, while the yield reached 250 mg uridine/g glucose.

CONCLUSION

This is the first report that more than 13 g uridine/l with a yield of 250 mg uridine/g glucose is produced in shake flask fermentation of genetically engineered Bacillus subtilis.

摘要

目的

为了提高尿苷的产量，对枯草芽孢杆菌中的尿苷产生代谢途径进行了修饰。

结果

将枯草芽孢杆菌 TD300 中 pur 操纵子的相对转录水平降低到 80%，衍生菌株 TD312 的产量增加到 11.81g 尿苷/L，产率增加到 270mg 尿苷/g 葡萄糖。通过 P 原位表达 pucR 基因，导致 pucR 基因的相对转录水平增加了 194.01 倍，ure 操纵子的相对转录水平增加了 349.71 倍。此外，TD314 的产量达到 13.06g 尿苷/L，产率达到 250mg 尿苷/g 葡萄糖。