Bukh A, Thomsen B S, Kimose H H, Nielsen L R, Svehag S E, Møller N P
Institute of Medical Microbiology, University of Aarhus, Denmark.
J Clin Lab Immunol. 1988 Aug;26(4):195-200.
A microplate-adapted polyclonal IgM-rheumatoid factor enzyme-linked immunosorbent assay (pIgM-RF ELISA) for the detection of circulating immune complexes (cIC) is presented. The assay involves the competitive binding of cIC and horseradish peroxidase conjugated aggregated human IgG (HRP-AHG) to solid-phase bound polyclonal IgM-RF (pIgM-RF). Aggregated human IgG (AHG) inhibited the binding of HRP-AHG to pIgM-RF in a dose-dependent way. The detection limit of the assay was about 125 ng AHG/ml diluted serum. The coefficients of variation for the assay varied from 5.0 to 14.7% for intra-assay runs and from 4.5 to 13.8% for inter-assay runs. The levels of cIC in sera from 29 patients with systemic lupus erythematosus (SLE), 85 untreated patients with breast cancer and 105 blood bank donors were studied by the pIgM-RF ELISA. Increased levels of cIC were demonstrated in 41.4% of the SLE group, in 8.2% of the breast cancer group, and in 1.9% of the normal control group. The difference in cIC activity between the SLE group and the normal control group was statistically significant.
本文介绍了一种用于检测循环免疫复合物（cIC）的微孔板适配多克隆IgM类风湿因子酶联免疫吸附测定法（pIgM-RF ELISA）。该测定法涉及cIC和辣根过氧化物酶偶联的聚集人IgG（HRP-AHG）与固相结合的多克隆IgM-RF（pIgM-RF）的竞争性结合。聚集人IgG（AHG）以剂量依赖方式抑制HRP-AHG与pIgM-RF的结合。该测定法的检测限约为125 ng AHG/ml稀释血清。该测定法的批内变异系数在5.0%至14.7%之间，批间变异系数在4.5%至13.8%之间。通过pIgM-RF ELISA研究了29例系统性红斑狼疮（SLE）患者、85例未经治疗的乳腺癌患者和105例血库献血者血清中的cIC水平。SLE组中41.4%、乳腺癌组中8.2%和正常对照组中1.9%的患者cIC水平升高。SLE组与正常对照组之间的cIC活性差异具有统计学意义。
