Center Excellence for Environmental Safety and Biological Effects, Beijing Key Laboratory for Green Catalysis and Separation, Department of Chemistry and Chemical Engineering, Beijing University of Technology, Beijing 100124, China.
Chem Commun (Camb). 2020 Feb 25;56(16):2423-2426. doi: 10.1039/c9cc09594b.
A highly sensitive laser-induced fluorescence detection system with real-time imaging focusing instead of the use of fluorescent reagents were developed for the detection of analytes in nanocapillaries. A cylindrical lens was adopted for the shaping of a laser beam to increase its spatial resolution. The limit of detection with a capillary having a radius of 440 nm was 6.76 yoctomoles (or four molecules) for fluorescein, and 84.5 yoctomoles (or 51 molecules) for FAM-labeled Hsa-miR-17. The designed system exhibited high sensitivity and stability for femtoliters of sample and was hence indicated to be suitable for analyses of single cells.
研究团队开发了一种高灵敏度的激光诱导荧光检测系统，该系统具有实时成像聚焦功能，无需使用荧光试剂即可检测纳毛细管中的分析物。研究人员采用柱面透镜对激光束进行整形，以提高其空间分辨率。对于半径为 440nm 的毛细管，检测限对于荧光素为 6.76 zeptomoles（或四个分子），对于 FAM 标记的 Hsa-miR-17 为 84.5 zeptomoles（或 51 个分子）。设计的系统对飞升级别的样品具有高灵敏度和稳定性，因此适用于单细胞分析。
