State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, China.
School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu Province, China.
Nat Prod Res. 2021 Nov;35(22):4740-4745. doi: 10.1080/14786419.2020.1715399. Epub 2020 Jan 29.
This study evaluated the antibacterial activities of different extracts of Gaertn. () on (). The extract solvent and procedure were screened, based on the yield of saponins and minimum inhibitory concentration (MIC). The results showed that the optimized product, fermentation and ethyl acetate extract by adding isoamyl alcohol from water extract of (SWFEAI), had the highest yield of saponins (7.83 ± 0.26%) and the best antibacterial activity (MIC = 0.125 mg/mL) on . The destroyed bacterial cell membrane and wall were observed by transmission electron microscopy, which then resulted in cell lysis and death. Furthermore, 20 compounds of SWFEAI were detected, among which oleanane-type triterpenoid saponins with molecular weights of 734, 750, 882, 924 and 966 were speculated to contribute to the antibacterial activities of SWFEAI. The results showed that SWFEAI could be a natural anti-acne agent.
本研究评估了 Gaertn. () 的不同提取物对 () 的抗菌活性。基于皂苷产率和最小抑菌浓度 (MIC)，筛选了提取溶剂和提取步骤。结果表明，从水提物 (SWFEAI) 中添加异戊醇的发酵和乙酸乙酯提取物是优化的产物，其皂苷产率最高（7.83±0.26%），对 的抗菌活性最好（MIC = 0.125 mg/mL）。透射电子显微镜观察到破坏的细菌细胞膜和细胞壁，导致细胞裂解和死亡。此外，从 SWFEAI 中检测到 20 种化合物，其中分子量为 734、750、882、924 和 966 的齐墩果烷型三萜皂苷被推测对 SWFEAI 的抗菌活性有贡献。结果表明，SWFEAI 可能是一种天然的抗粉刺剂。
