Department of Rehabilitation Medicine, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, Chengdu, China.
Department of Neurology, Affiliated Hospital of Southwest Medical University, Luzhou, China.
Acupunct Med. 2020 Jun;38(3):175-180. doi: 10.1177/0964528419882937. Epub 2020 Jan 30.
To evaluate the effects of electroacupuncture (EA) combined with exercise training on motor function and microtubule-associated protein (MAP)-2 in the hippocampal CA3 region of rats in the middle and late stages of cerebral infarction, and explore potential underlying mechanisms of action.
A total of 80 Wistar rats were randomly divided into model, EA, training and EA + training groups (n = 20 per group) after establishing the middle cerebral artery occlusion (MCAO) model of cerebral infarction. Rats were treated with EA and/or training in the sixth week post-MCAO. After receiving 2 weeks of treatment, motor function was assessed and MAP-2 expression in the CA3 region was measured using an immunohistochemical method.
Compared to the model group, significant differences in walking stick, balance beam and screen capture ability were detected in the EA, training and EA + training groups (p < 0.05). The EA + training group showed greater improvements than the EA and training groups (p < 0.05 each). Significant differences in MAP-2 expression were detected in the EA, training and EA + training groups compared to the model group (p < 0.05). MAP-2 expression was higher in the EA + training group than in the EA and training groups (p < 0.05 each).
MAP-2 expression and motor functional recovery were higher in the combined therapy (EA + training) group compared to the monotherapy (EA or training) groups. EA combined with exercise training appeared to significantly promote the recovery of motor function in the middle and late stages of cerebral infarction in this rat model.
评估电针（EA）联合运动训练对脑梗死中后期大鼠海马 CA3 区运动功能和微管相关蛋白（MAP）-2的影响，并探讨其潜在的作用机制。
80 只 Wistar 大鼠建立大脑中动脉闭塞（MCAO）脑梗死模型后，随机分为模型组、EA 组、训练组和 EA+训练组（每组 20 只）。MCAO 后第 6 周开始给予 EA 和/或训练。治疗 2 周后，采用步态分析、平衡木和屏幕抓握能力检测大鼠运动功能，免疫组化法检测海马 CA3 区 MAP-2 表达。
与模型组比较，EA 组、训练组和 EA+训练组大鼠步杖、平衡木和屏幕抓握能力均显著改善（p<0.05），且 EA+训练组优于 EA 组和训练组（p<0.05 各）。与模型组比较，EA 组、训练组和 EA+训练组大鼠海马 CA3 区 MAP-2 表达均显著升高（p<0.05），且 EA+训练组高于 EA 组和训练组（p<0.05 各）。
与单疗法（EA 或训练）组相比，联合疗法（EA+训练）组大鼠 MAP-2 表达和运动功能恢复更好。电针联合运动训练可显著促进该脑梗死大鼠模型中后期运动功能的恢复。
