Sysoev Yuriy I, Prikhodko Veronika A, Kan Aleksandra V, Titovich Irina A, Karev Vadim E, Okovityi Sergey V
Department of Pharmacology and Clinical Pharmacology, Saint Petersburg State Chemical and Pharmaceutical University, 197022 Saint Petersburg, Russia.
Laboratory of Neuroprosthetics, Institute of Translational Biomedicine, Saint Petersburg State University, 199034 Saint Petersburg, Russia.
Neurol Int. 2022 Jun 21;14(3):547-560. doi: 10.3390/neurolint14030044.
Ischemic stroke is a leading cause of death and disability worldwide. To search for new therapeutic and pharmacotherapeutic strategies, numerous models of this disease have been proposed, the most popular being transient middle cerebral artery occlusion. Behavioral and sensorimotor testing, biochemical, and histological methods are traditionally used in conjunction with this model to assess the effectiveness of potential treatment options. Despite its wide overall popularity, electroencephalography/electrocorticography is quite rarely used in such studies. In the present work, we explored the changes in brain electrical activity at days 3 and 7 after 30- and 45-min of transient middle cerebral artery occlusion in rats. Cerebral ischemia altered the amplitude and spectral electrocorticogram characteristics, and led to a reorganization of inter- and intrahemispheric functional connections. Ischemia duration affected the severity as well as the nature of the observed changes. The dynamics of changes in brain electrical activity may indicate a spontaneous partial recovery of impaired cerebral functions at post-surgery day 7. Our results suggest that electrocorticography can be used successfully to assess the functional status of the brain following ischemic stroke in rats as well as to investigate the dynamics of functional recovery.
缺血性中风是全球范围内导致死亡和残疾的主要原因。为了寻找新的治疗和药物治疗策略，人们提出了许多该疾病的模型，其中最常用的是短暂性大脑中动脉闭塞模型。传统上，行为和感觉运动测试、生化和组织学方法与该模型结合使用，以评估潜在治疗方案的有效性。尽管该模型总体上广受欢迎，但脑电图/皮质电图在这类研究中很少使用。在本研究中，我们探讨了大鼠短暂性大脑中动脉闭塞30分钟和45分钟后第3天和第7天脑电活动的变化。脑缺血改变了皮质电图的振幅和频谱特征，并导致半球间和半球内功能连接的重组。缺血持续时间影响了观察到的变化的严重程度和性质。脑电活动变化的动态过程可能表明术后第7天受损脑功能有自发的部分恢复。我们的结果表明，皮质电图可成功用于评估大鼠缺血性中风后脑的功能状态，以及研究功能恢复的动态过程。
