Laboratory of Theriogenology, Department of Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Laboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
J Reprod Dev. 2020 Apr 10;66(2):149-154. doi: 10.1262/jrd.2019-148. Epub 2020 Jan 28.
Epidermal growth factor (EGF) concentrations in the uterus show two peaks on days 2-4 and 13-14 during the estrous cycle in fertile cows. Loss of the two peaks has been linked to reduced fertility in repeat breeder cows. This study aimed to examine the effect of seminal plasma (SP) on normalizing endometrial EGF concentrations and restoring fertility in repeat breeder cows with low EGF concentrations on day 3. In study 1, we examined the effect of the deposition sites (the vagina and uterus) of SP on the endometrial EGF concentrations in repeat breeder cows. SP infusion into the vagina, but not uterus, on the first day of the estrus cycle (day 0) normalized the endometrial EGF concentrations (≥ 4.7 ng/g tissue weight) on day 3. In study 2, the effect of SP volume (0.5 and 10 ml of SP and 0.5 ml of SP diluted to 10 ml) on EGF concentrations was examined. All groups with SP infusion had increased EGF concentrations on day 3, and cows with 10 ml of SP and 0.5 ml of SP diluted to 10 ml showed the highest levels of EGF concentrations. In study 3, we examined the effect of SP infusion on fertility. SP infusion normalized two peaks of endometrial EGF concentrations in about 60% of repeat breeder cows and produced more pregnancies than the controls (44.4 vs. 19.4%). Therefore, we concluded that SP may contain an activity to normalize the EGF profile and restore fertility in repeat breeder cows with altered EGF profiles.
发情周期中，生育力正常的奶牛子宫内的表皮生长因子（EGF）浓度在第 2-4 天和第 13-14 天出现两个峰值。两个峰值的缺失与重复配种奶牛生育力下降有关。本研究旨在探讨精液（SP）对第 3 天 EGF 浓度较低的重复配种奶牛的子宫内膜 EGF 浓度正常化和生育力恢复的影响。在研究 1 中，我们检测了 SP 的沉积部位（阴道和子宫）对重复配种奶牛子宫内膜 EGF 浓度的影响。在发情周期的第一天（第 0 天）将 SP 注入阴道而非子宫，可使第 3 天的子宫内膜 EGF 浓度（≥4.7ng/g 组织重量）正常化。在研究 2 中，我们检测了 SP 体积（0.5 和 10 ml SP 以及 0.5 ml SP 稀释至 10 ml）对 EGF 浓度的影响。所有 SP 输注组在第 3 天的 EGF 浓度均增加，输注 10 ml SP 和 0.5 ml SP 稀释至 10 ml 的牛的 EGF 浓度最高。在研究 3 中，我们检测了 SP 输注对生育力的影响。SP 输注可使约 60%的重复配种奶牛的子宫内膜 EGF 浓度的两个峰值正常化，并产生比对照组更多的妊娠（44.4%对 19.4%）。因此，我们得出结论，SP 可能含有一种使改变的 EGF 谱正常化并恢复重复配种奶牛生育力的活性。
