Research Group of Industrial Microbiology and Food Biotechnology (IMDO), Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Brussels, Belgium.
Food Microbiol. 2020 Jun;88:103402. doi: 10.1016/j.fm.2019.103402. Epub 2019 Dec 27.
Recently, a metagenomic study of a water kefir fermentation ecosystem enabled the reconstruction of a metagenome-assembled genome (MAG) of an Oenococcus species that was different from the three species of this genus known so far. Therefore, the name Candidatus Oenococcus aquikefiri was proposed for this novel Oenococcus species. In the meantime, however, a fourth member of the genus, Oenococcus sicerae, isolated from French cider, was reported. The comparison of its genome sequence with the Candidatus O. aquikefiri MAG showed an average nucleotide identity (ANI) value of 98.53%. In addition, the 16S rRNA and pheS genes of the two species were 99.4% and 99.9% identical, respectively. As the presence of O. sicerae in a water kefir metagenome was also revealed by metagenomic recruitment plotting, it can be stated that Candidatus O. aquikefiri and O. sicerae belong to the same species. Intraspecies variations include the presence or absence of a citrate lyase operon and components of various phosphotransferase (PTS) transport systems.
最近，一项对水克菲尔发酵生态系统的宏基因组研究，使得重建了一个不同于迄今为止已知的三个属种的，属于琼胶乳球菌属（Oenococcus）的微生物宏基因组组装基因组（MAG）。因此，为这个新型琼胶乳球菌属种提议了一个暂用名“Aquikefiri 海洋琼胶乳球菌”。与此同时，一个来自法国苹果酒的琼胶乳球菌属第四种，Oenococcus sicerae，也被报道。将其基因组序列与“Candidatus O. aquikefiri”MAG 进行比较，发现平均核苷酸同一性（ANI）值为 98.53%。此外，这两个物种的 16S rRNA 和 pheS 基因分别有 99.4%和 99.9%的同一性。由于水克菲尔宏基因组中也存在 O. sicerae，通过宏基因组募集绘图可以推断，“Candidatus O. aquikefiri”和 O. sicerae 属于同一个种。种内变异包括柠檬酸裂解酶基因簇的存在或缺失，以及各种磷酸转移酶（PTS）转运系统的组成成分。
