Normandie Univ, UNICAEN, UNIROUEN, ABTE, 14000 Caen, France.
Normandie Univ, UNICAEN, SF ICORE 4206, CMAbio3, F-14032 Caen, France.
Syst Appl Microbiol. 2019 May;42(3):302-308. doi: 10.1016/j.syapm.2018.12.006. Epub 2018 Dec 21.
Two Gram-stain-positive, small ellipsoidal cocci, non-motile, oxidase- and catalase-negative, and facultative anaerobic strains (UCMA15228 and UCMA17102) were isolated in France, from fermented apple juices (ciders). The 16S rRNA gene sequence was identical between the two isolates and showed 97 % similarity with respect to the closest related species Oenococcus oeni and O. kitaharae. Therefore, the two isolates were classified within the genus Oenococcus. The phylogeny based on the pheS gene sequences also confirmed the position of the new taxon. DNA-DNA hybridizations based on in silico genome-to-genome comparisons (GGDC) and Average Nucleotide Identity (ANI) values, as well as species-specific PCR, validated the novelty of the taxon. Various phenotypic characteristics such as the optimum temperature and pH for growth, the ability to metabolise sugars, the aptitude to perform the malolactic fermentation, and the resistance to ethanol and NaCl, revealed that the two strains are distinguishable from the other members of the Oenococcus genus. The combined genotypic and phenotypic data support the classification of strains UCMA15228 and UCMA17102 into a novel species of Oenococcus, for which the name O. sicerae sp. nov. is proposed. The type strain is UCMA15228 (=DSM107163=CIRM-BIA2288).
两株革兰氏阳性、小椭圆形球菌，不运动，氧化酶和过氧化氢酶阴性，兼性厌氧菌株（UCMA15228 和 UCMA17102）于法国从发酵苹果汁（苹果酒）中分离得到。两株分离株的 16S rRNA 基因序列完全相同，与最接近的种片球菌属和奥奈库姆片球菌属的亲缘关系最近，相似度为 97%。因此，两株分离株被分类在片球菌属内。基于 pheS 基因序列的系统发育也证实了新分类单元的地位。基于基因组到基因组比较（GGDC）和平均核苷酸同一性（ANI）值的 DNA-DNA 杂交以及种特异性 PCR，验证了该分类单元的新颖性。各种表型特征，如最适生长温度和 pH 值、糖代谢能力、进行苹果酸-乳酸发酵的能力以及对乙醇和 NaCl 的抗性，表明这两株菌株与片球菌属的其他成员不同。综合表型和基因型数据支持将菌株 UCMA15228 和 UCMA17102 分类为片球菌属的一个新种，建议将其命名为 O. sicerae sp. nov.。模式菌株为 UCMA15228（=DSM107163=CIRM-BIA2288）。
