Alam Prawez, Kamal Y T, Alqasoumi Saleh Ibrahim, Foudah Ahmed Ibrahim, Alqarni Mohammed Hamed, Yusufoglu Hasan Soliman
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.
Department of Pharmacognosy, College of Pharmacy, King Khalid University, Abha 61471, Saudi Arabia.
Saudi Pharm J. 2019 Nov;27(7):975-980. doi: 10.1016/j.jsps.2019.07.006. Epub 2019 Jul 16.
A new rapid, simple, sensitive and high performance thin layer chromatography (HPTLC) has been established for the simultaneous determination of ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice and herbal formulation. HPTLC method was carried out using ethyl acetate: acetone: water: formic acid, 10:6:2:2 (%, )) on 20 × 10 cm glass coated silica gel 60 F plates and scanned at 254 nm for ascorbic acid and gallic acid. Ascorbic acid and gallic acid in the freeze-dried pomegranate fruit juice were identified by comparing their single spot at R = 0.54 ± 0.02 and R = 0.83 ± 0.01 respectively. The value of regression equation (r ≥ 0.9992) revealed a good linear relationship between peak area and amount of ascorbic acid and gallic acid in the range of 100-800 ng/band. The method was validated for precision, accuracy, robustness LOD and LOQ. The method proposed can be useful for routine determination of ascorbic acid and gallic acid in various crude as well as herbal formulations as a quality control tool.
已建立一种新的快速、简便、灵敏且高效的薄层色谱法（HPTLC），用于同时测定冻干石榴果汁和草药制剂中的抗坏血酸和没食子酸。HPTLC法采用乙酸乙酯∶丙酮∶水∶甲酸，10∶6∶2∶2（%，v/v），在20×10 cm玻璃涂布硅胶60 F板上进行，对抗坏血酸和没食子酸在254 nm处进行扫描。通过比较冻干石榴果汁中抗坏血酸和没食子酸的单点，分别在Rf = 0.54±0.02和Rf = 0.83±0.01处对其进行鉴定。回归方程的值（r≥0.9992）表明，在100 - 800 ng/条带范围内，峰面积与抗坏血酸和没食子酸的量之间具有良好的线性关系。该方法在精密度、准确度、稳健性、检测限和定量限方面得到了验证。所提出的方法作为一种质量控制工具可用于常规测定各种粗提物以及草药制剂中的抗坏血酸和没食子酸。
