Alam Prawez, Foudah Ahmed I, Zaatout Hala H, T Kamal Y, Abdel-Kader Maged S
Department of Pharmacognosy, College of Pharmacy, Prince Sattam Bin Abdulaziz University, P.O. Box 173, Al-Kharj 11942, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Alexandria University, Alexandria 21215, Egypt.
Afr J Tradit Complement Altern Med. 2017 Jan 13;14(2):198-205. doi: 10.21010/ajtcam.v14i2.21. eCollection 2017.
A simple and sensitive thin-layer chromatographic method has been established for quantification of glycyrrhizin in rhizome and baby herbal formulations by validated Reverse Phase HPTLC method.
RP-HPTLC Method was carried out using glass coated with RP-18 silica gel 60 F254S HPTLC plates using methanol-water (7: 3 v/v) as mobile phase.
The developed plate was scanned and quantified densitometrically at 256 nm. Glycyrrhizin peaks from rhizome and baby herbal formulations were identified by comparing their single spot at R = 0.63 ± 0.01. Linear regression analysis revealed a good linear relationship between peak area and amount of glycyrrhizin in the range of 2000-7000 ng/band.
The method was validated, in accordance with ICH guidelines for precision, accuracy, and robustness. The proposed method will be useful to enumerate the therapeutic dose of glycyrrhizin in herbal formulations as well as in bulk drug.
已通过经过验证的反相高效薄层色谱法建立了一种简单灵敏的薄层色谱法，用于定量根茎和婴儿草药制剂中的甘草酸。
采用RP - HPTLC法，使用涂有RP - 18硅胶60 F254S的高效薄层板，以甲醇 - 水（7:3 v/v）为流动相。
展开后的薄板在256 nm处进行扫描和密度测定定量。通过比较根茎和婴儿草药制剂中甘草酸峰在Rf = 0.63±0.01处的单点，鉴定出甘草酸峰。线性回归分析表明，在2000 - 7000 ng/条带范围内，峰面积与甘草酸含量之间存在良好的线性关系。
该方法按照ICH指南进行了精密度、准确度和稳健性验证。所提出的方法将有助于确定草药制剂以及原料药中甘草酸的治疗剂量。
