Nakatsuchi Ayumi, Inagaki Mizuho, Sugiyama Michiyo, Usui Masaru, Asai Tetsuo
Faculty of Applied Biological Science, Gifu University, 1-1 Yanadigo, Gifu 501-1193, Japan.
United Graduate School of Veterinary Science, Gifu University, 1-1 Yanadigo, Gifu 501-1193, Japan.
Food Saf (Tokyo). 2018 Dec 21;6(4):156-159. doi: 10.14252/foodsafetyfscj.2018012. eCollection 2018 Dec.
Fluoroquinolone is widely used for the treatment of bacterial diseases, and the emergence of quinolone resistance has become a serious concern in recent years, owing to an increase and inappropriate use of antimicrobials. Here, we attempted to understand the differences in the emergence frequency of quinolone-resistant bacterial variants in three serotypes . Infantis, . Schwarzengrund, and . Manhattan-which are mainly found in broiler industries in Japan. Emergence frequency tests for quinolone-resistant variants using enrofloxacin-containing agar plates and sequence analysis in the quinolone resistance-determining region (QRDR) of in DNA gyrase were performed. The results showed no significant difference in the emergence frequency among the three serotypes, and most of the resistant variants had mutations in the QRDR region. These findings suggest that differences in the serotypes tested are not associated with the emergence frequency of quinolone-resistant variants.
氟喹诺酮广泛用于治疗细菌疾病，近年来，由于抗菌药物的使用增加和不当使用，喹诺酮耐药性的出现已成为一个严重问题。在此，我们试图了解三种血清型（婴儿型、施瓦岑格鲁德型和曼哈顿型，这些血清型主要在日本的肉鸡行业中发现）的喹诺酮耐药细菌变体出现频率的差异。使用含恩诺沙星的琼脂平板进行喹诺酮耐药变体的出现频率测试，并对DNA促旋酶喹诺酮耐药决定区（QRDR）进行序列分析。结果显示三种血清型之间的出现频率没有显著差异，并且大多数耐药变体在QRDR区域有突变。这些发现表明，所测试血清型的差异与喹诺酮耐药变体的出现频率无关。
