Division of Pathology and Bacteriology, Fukuoka Institute of Health and Environmental Sciences, Dazaifu, Japan.
Foodborne Pathog Dis. 2010 Jun;7(6):727-35. doi: 10.1089/fpd.2009.0438.
The study sets out to either confirm or refute a recent study's findings that chicken meat is an unlikely source of Salmonella enterica subspecies enterica serovar Infantis (Salmonella Infantis) in humans in the Kyushu-Okinawa region, Japan.
A total of 74 Salmonella Infantis isolates (30 from human and 44 from other sources), mainly from the Kyushu-Okinawa region in south-western Japan, were analyzed using a molecular-epidemiological approach combining two fingerprinting methods, namely pulsed-field gel electrophoresis and amplified fragment length polymorphism (AFLP), a novel polymerase chain reaction-based technique.
The resulting pulsed-field profiles showed that 17 of 30 human isolates were similar to those found in chicken meat, whereas there were no common pulsed-field profiles between human and chicken egg isolates. Overall, 3 of 18 AFLP profiles included 7 human isolates and 14 chicken egg isolates. In addition, the combined results of the pulsed-field gel electrophoresis and AFLP analyses showed that 8 human Salmonella Infantis and 13 chicken meat isolates belonged to the same clonal lines.
These results suggest that chicken meat is an infection source of Salmonella Infantis for humans in the Kyushu-Okinawa region, Japan. The results also showed the relatively high suitability of AFLP for application to epidemiological studies of Salmonella Infantis.
本研究旨在证实或反驳最近的一项研究结果，即在日本九州-冲绳地区，鸡肉不太可能是人类感染肠炎沙门氏菌亚种肠炎亚种（肠炎沙门氏菌）的来源。
采用分子流行病学方法，结合两种指纹图谱分析技术，即脉冲场凝胶电泳和扩增片段长度多态性（AFLP），对主要来自日本西南部九州-冲绳地区的 74 株肠炎沙门氏菌分离株（30 株来自人类，44 株来自其他来源）进行分析。
所得的脉冲场图谱显示，30 株人类分离株中有 17 株与鸡肉中发现的分离株相似，而人类和鸡蛋分离株之间没有共同的脉冲场图谱。总体而言，18 种 AFLP 图谱中有 3 种包含 7 株人类分离株和 14 株鸡蛋分离株。此外，脉冲场凝胶电泳和 AFLP 分析的综合结果表明，8 株人类肠炎沙门氏菌和 13 株鸡肉分离株属于同一克隆系。
这些结果表明，鸡肉是日本九州-冲绳地区人类感染肠炎沙门氏菌的感染源。结果还表明，AFLP 非常适合用于肠炎沙门氏菌的流行病学研究。
