Wang Jiajia, Li Jitao, Ge Qianqian, Chen Zhao, Li Jian
Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture and Rural, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, China.
Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Int J Genomics. 2020 Jan 3;2020:5735968. doi: 10.1155/2020/5735968. eCollection 2020.
The could be a useful crustacean laboratory animal in many research fields. We newly established an inbred line of named EC4 inbred line by brother×sister mating and keeping to generation. Trends in heterozygosity in the process of producing EC4 inbred line were examined through the characterization of polymorphisms based on gene frequencies of SNP and EST-SSR loci. The results demonstrated that the number of alleles (), observed heterozygosity (), expected heterozygosity (), and polymorphism information content (PIC) gradually decreased with the increase of inbreeding generations. The genetic detection results indicated that 9 (29.03%, 9/31) of the SNP loci and 15 (32.61%, 15/46) of the EST-SSR loci were homozygous in generation of EC4 inbred line. The variation of the growth-related traits, the immune responses, and antioxidant status were described in experimental full-sibling inbred populations of at five levels of inbreeding coefficient ( = 0.785, = 0.816, = 0.859, = 0.886, = 0.908) under controlled laboratory conditions. The body weight, body length, and survival rate in EC4 inbred line of all generations were less than the control population. Inbreeding affected the antibacterial activity, phenoloxidase (PO) activity, and superoxide dismutase (SOD) which decreased at the eleventh generation of EC4 inbred line. This study demonstrated that inbreeding had a negative effect on the economic traits and immune response, but our inbred line was established successfully until and confirmed by genetic detection using SNP and EST-SSR loci.
在许多研究领域中，[具体甲壳类动物名称未给出]可能是一种有用的甲壳类实验动物。我们通过兄妹交配并保持到[具体代数未给出]代，新建立了一个名为EC4近交系的[具体甲壳类动物名称未给出]近交系。通过基于SNP和EST-SSR位点基因频率的多态性表征，研究了EC4近交系产生过程中的杂合度趋势。结果表明，随着近交代数的增加，等位基因数（）、观察杂合度（）、期望杂合度（）和多态性信息含量（PIC）逐渐降低。遗传检测结果表明，在EC4近交系的[具体代数未给出]代中，9个（29.03%，9/31）SNP位点和15个（32.61%，15/46）EST-SSR位点是纯合的。在受控实验室条件下，描述了[具体甲壳类动物名称未给出]实验全同胞近交群体在五个近交系数水平（ = 0.785， = 0.816， = 0.859， = 0.886， = 0.908）下生长相关性状、免疫反应和抗氧化状态的变化。所有世代的EC4近交系的体重、体长和存活率均低于对照群体。近交影响了抗菌活性、酚氧化酶（PO）活性和超氧化物歧化酶（SOD），这些在EC4近交系的第十一代有所下降。本研究表明，近交对经济性状和免疫反应有负面影响，但我们的近交系成功建立到[具体代数未给出]代，并通过使用SNP和EST-SSR位点的遗传检测得到证实。
