Plant Biochemistry Department, National Research Centre (NRC), 33 El Bohouth St. (Former El-Tahrir St.), 12622 Dokki, Giza, Egypt.
Pharmacology Department, National Research Centre (NRC), 33 El Bohouth St. (Former El-Tahrir St.), 12622 Dokki, Giza, Egypt.
Biomed Res Int. 2020 Jan 2;2020:1295492. doi: 10.1155/2020/1295492. eCollection 2020.
Diabetic neuropathy (DN) is a widespread disabling disorder including peripheral nerves' damage. The aim of the current study was to estimate the potential ameliorative effect of on DN and the involvement of the thioredoxin. Diabetes was induced by streptozotocin (STZ; 50 mg/kg; i.p). Glimepiride (0.5 mg/kg) or powder (100 or 200 mg/kg) were given orally, after 2 days of STZ injection for 4 weeks. Glucose, total antioxidant capacity (TAC), superoxide dismutase (SOD), and catalase (CAT) serum levels as well as brain contents of thioredoxin (Trx), tumor necrosis factor-alpha (TNF-), and interleukin-6 (IL-6) were measured with the histopathological study. STZ-induced DN resulted in a significant ( < 0.05) rise in glucose blood level and brain contents of TNF- and IL-6 and produced a reduction in serum TAC, SOD, CAT, and brain Trx levels with irregular islets of Langerhans cells and loss of brain Purkinje cells. Treatment with glimepiride or both doses of alleviated these biochemical and histological parameters as compared to the STZ group. has a neurotherapeutic effect against DN via its inhibitory effect on inflammatory mediators and oxidative stress molecules with its upregulation of Trx activity.
糖尿病性神经病（DN）是一种广泛的致残性疾病，包括周围神经损伤。本研究的目的是评估对 DN 的潜在改善作用及其与硫氧还蛋白的关系。糖尿病通过链脲佐菌素（STZ；50mg/kg；腹腔注射）诱导。在 STZ 注射后 2 天，给予格列美脲（0.5mg/kg）或 粉末（100 或 200mg/kg）口服，连续 4 周。用组织病理学研究测定血清中葡萄糖、总抗氧化能力（TAC）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）水平以及脑中硫氧还蛋白（Trx）、肿瘤坏死因子-α（TNF-）和白细胞介素-6（IL-6）的含量。STZ 诱导的 DN 导致血糖水平显著升高（<0.05），脑中 TNF-和 IL-6 含量增加，血清 TAC、SOD、CAT 和脑中 Trx 水平降低，胰岛朗格汉斯细胞不规则，脑浦肯野细胞丢失。与 STZ 组相比，格列美脲或 两种剂量的治疗均可改善这些生化和组织学参数。通过抑制炎症介质和氧化应激分子，以及上调 Trx 活性，对 DN 具有神经治疗作用。
