Beijing Advanced Innovation Centre for Food Nutrition and Human Health, Beijing Key Laboratory of Flavour Chemistry, Beijing Technology and Business University, Beijing, China.
Luminescence. 2020 Aug;35(5):754-762. doi: 10.1002/bio.3781. Epub 2020 Jan 30.
A dual-function fluorescent probe (Probe 1) was developed for discriminative detection of Hg and N H . Probe 1 could discriminatively detect Hg and N H through two different reaction sites, with the mechanism for Probe 1 for Hg depending on a desulfurization reaction and for N H depending on the Schiff-base reaction. N H had minimal effect on Hg detection in dimethyl sulfoxide (DMSO)/H O solution, but Hg could interfere with N H detection in DMSO/buffer solution. Different concentrations of Hg and N H resulted in different blue shades of Probe 1 test strips, and the shade of blue was different with the same concentration of Hg or N H , as observed under ultraviolet light at 365 nm wavelength.
一种双功能荧光探针(探针 1)被开发用于区分检测 Hg 和 NH。探针 1 可以通过两个不同的反应位点来区分检测 Hg 和 NH,其检测 Hg 的机制依赖于脱硫反应,而检测 NH 则依赖于希夫碱反应。NH 对 DMSO/H2O 溶液中 Hg 检测的影响很小,但 Hg 可以干扰 DMSO/缓冲溶液中 NH 检测。不同浓度的 Hg 和 NH 导致探针 1 试条呈现不同的蓝色阴影,并且在 365nm 波长的紫外光下观察到,相同浓度的 Hg 或 NH 的蓝色阴影不同。