C3HC 型锌指蛋白（ZFC3）与 Lon/MAP1 的相互作用对于调控线粒体基因、感染菌丝发育和稻瘟病菌的寿命很重要。

The C3HC type zinc-finger protein (ZFC3) interacting with Lon/MAP1 is important for mitochondrial gene regulation, infection hypha development and longevity of Magnaporthe oryzae.

机构信息

College of Plant Sciences, Jilin University, Changchun, China.

Present address: Institute of Phytopathology, Centre for BioSystems, Land Use and Nutrition, Justus Liebig University, Heinrich Buff-Ring 26-32, D-35392, Giessen, Germany.

出版信息

BMC Microbiol. 2020 Jan 30;20(1):23. doi: 10.1186/s12866-020-1711-4.

BACKGROUND

The rice blast is a typical fungal disease caused by Magnaporthe oryzae, and the mitochondrial ATP-dependent Lon protease (MAP1) has been proven to be involved in blast development. We previously screened a C3HC type Zinc-finger domain protein (ZFC3), which is interacted with MAP1. The purpose of this research was to study the biological function of ZFC3 protein in M. oryzae.

RESULTS

We first confirmed that the ZFC3-RFP fusion protein is localized within the mitochondria. The deleted mutant strains of ZFC3 (∆ZFC3) showed the enhanced expression level of mtATP6, particularly mtATP8, and almost unchanged nATP9. ΔZFC3 produces more conidia and more tolerance to multiple stressors. The knock-out strain shows more melanin accumulation suggests the susceptibility to aging. ΔZFC3 displays faster early-stage hypha infiltration involved in MAP1-mediated pathogenicity in host rice.

CONCLUSION

These results support the view that ZFC3 is a key regulator involved in gene regulation, stress response, cell wall integrity, longevity, conidiation, infection hypha development and MAP1-mediated pathogenicity in M. oryzae.

背景

稻瘟病是由稻瘟病菌引起的一种典型真菌病害，线粒体 ATP 依赖型 Lon 蛋白酶（MAP1）已被证明参与了稻瘟病的发展。我们之前筛选出了与 MAP1 相互作用的 C3HC 型锌指结构域蛋白（ZFC3）。本研究旨在研究 ZFC3 蛋白在稻瘟病菌中的生物学功能。

结果

我们首先证实 ZFC3-RFP 融合蛋白定位于线粒体中。ZFC3 的缺失突变株（∆ZFC3）表现出 mtATP6，特别是 mtATP8 的表达水平增强，而 nATP9 几乎不变。∆ZFC3 产生更多的分生孢子，对多种胁迫源的耐受性更强。敲除株系表现出更多的黑色素积累，表明其对衰老的敏感性增加。∆ZFC3 显示出更快的早期菌丝渗透，这与 MAP1 介导的宿主水稻致病性有关。