Taguchi H, Tanaka R, Nishimura K, Miyaji M
Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Japan.
Mycopathologia. 1988 Aug;103(2):87-90. doi: 10.1007/BF00441263.
We applied a flow cytometry apparatus (FCM) to differentiating Exophiala dermatitidis, E. moniliae and E. jeanselmei from each other. The wavelength of the argon laser emitted from the FCM was 488 nm and the aperture of nozzle from which the stream of fluid containing single cells was blown out was 100 micron. By irradiating the stream with laser by either the forward light scatter (FLS) or by the perpendicular light scatter (PLS), we were able to get two pieces of informations. Histograms displayed by the FLS indicate the cell size, while dot displays by the PLS reflect the cell structure. As a result, E. dermatitidis was clearly differentiated from either E. moniliae or E. jeanselmei by their histograms by FLS. In addition, dot displays by the PLS differentiated E. moniliae from E. jeanselmei. In conclusion, flow cytometry is available for differentiating E. dermatitidis, E. moniliae and E. jeanselmei from each other.
我们应用流式细胞仪（FCM）来区分皮炎外瓶霉、念珠菌样外瓶霉和甄氏外瓶霉。FCM发射的氩激光波长为488nm，吹出含单细胞流体流的喷嘴孔径为100微米。通过前向光散射（FLS）或垂直光散射（PLS）用激光照射流体流，我们能够获得两条信息。FLS显示的直方图表明细胞大小，而PLS的点图反映细胞结构。结果，通过FLS的直方图，皮炎外瓶霉能够与念珠菌样外瓶霉或甄氏外瓶霉明显区分开来。此外，PLS的点图将念珠菌样外瓶霉与甄氏外瓶霉区分开来。总之，流式细胞术可用于区分皮炎外瓶霉、念珠菌样外瓶霉和甄氏外瓶霉。
