Bansal Yashik, Aggarwal Alisha, Gadepalli Ravisekhar, Nag Vijaya Lakshmi
Department of Microbiology, All India Institute of Medical Sciences, Jodhpur, Rajasthan, India.
Indian J Med Microbiol. 2019 Jul-Sep;37(3):426-432. doi: 10.4103/ijmm.IJMM_19_358.
Brucellosis is a significant bacterial zoonotic disease with a high seroprevalence in low-to-middle-income countries where there is a significant contact of humans with animals. This prospective study was conducted to observe the seroprevalence of brucellosis in 75 symptomatic patients with pyrexia of unknown origin (PUO) and 75 high-risk individuals (10 veterinarians, 15 milkmen and 50 healthy contacts of symptomatic patients) with possible exposure to brucellosis. Serum samples collected from these patients and individuals were subjected to rose Bengal test (RBT) and ELISA for the detection of IgM and IgG antibodies. RBT was positive in 50 samples (40 PUO and 10 high risk), of which 25 (33.3%) PUO cases had detectable IgM antibodies, whereas IgG antibodies were detected in 20 patients. None of samples from high-risk individuals was reactive for IgM/IgG antibodies by ELISA. The overall seroprevalence in this study was 16.7% (33.3% in PUO patients). Being a predominantly livestock rearing area, brucellosis is a significant health-care problem in this part of India with this entity being linked to 33% of PUO cases.
布鲁氏菌病是一种重要的细菌性人畜共患病，在人与动物有大量接触的中低收入国家血清阳性率很高。本前瞻性研究旨在观察75例不明原因发热（PUO）的有症状患者及75名可能接触布鲁氏菌病的高危个体（10名兽医、15名挤奶工和50名有症状患者的健康接触者）中布鲁氏菌病的血清阳性率。从这些患者和个体采集的血清样本进行了玫瑰红试验（RBT）以及用于检测IgM和IgG抗体的ELISA检测。RBT在50份样本中呈阳性（40例PUO患者和10名高危个体），其中25例（33.3%）PUO病例可检测到IgM抗体，而20例患者检测到IgG抗体。高危个体的样本通过ELISA检测IgM/IgG抗体均无反应性。本研究中的总体血清阳性率为16.7%（PUO患者中为33.3%）。作为一个主要的畜牧养殖地区，布鲁氏菌病在印度的这一地区是一个重大的卫生保健问题，该疾病与33%的PUO病例有关。
