Fiber endface illumination diffuser for endo-cavity photoacoustic imaging.

The laser illumination delivery method is important in designing probes that achieve high imaging quality and deep tissue penetration. Here we present a novel, to the best of our knowledge, fiber diffuser tip using microspheres dispersed within an ultraviolet adhesive to scatter light. This diffuser keeps the skin surface fluence under the maximum permissible exposure, while enabling higher laser energy injection to enhance the photoacoustic (PA) signal generated from the tissue. We compare the light diffusion effects of different microsphere materials, sizes, and concentrations, and find that 10 µm silica microspheres provide the best light scattering with minimal 5% output energy loss. With the Zemax simulation and experimental validation, we show that this fiber diffuser tip is a valuable tool for endo-cavity PA imaging.