Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, China.
Department of Forestry Protection, School of Forestry and Biotechnology, Zhejiang A&F University, 666 Wusu street, Linan, Hangzhou, Zhejiang 311300, China.
Int J Biol Macromol. 2020 May 1;150:200-205. doi: 10.1016/j.ijbiomac.2020.01.271. Epub 2020 Jan 28.
Sciaridae is a family of great species diversity, distributed worldwide, that includes important agricultural pests of cultivated mushrooms and plants produced in greenhouses. Here we sequenced five nearly complete mitochondrial genomes representing three subfamilies of Sciaridae. The lengths of these mitogenomes range from 13,849 bp to 16,923 bp with 13 protein-coding genes (PCGs), 20-22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, and a control region (CR). Compared with other dipteran species, rearrangements in Sciaridae are more common. Inversion or transition is observed frequently of trnL2, and in the tRNA clusters trnI-trnQ-trnM, trnW-trnC-trnY, and trnA-trnR-trnN-trnS1-trnE-trnF. Phylogenetic relationships within the family were reconstructed based on these newly sequenced species, combined with the published mitogenomes of related families, and recovered the topology within Sciaroidea as Cecidomyiidae + (Sciaridae + Keroplatidae). Relationships recovered within Sciaridae were Sciarinae + ('Pseudolycoriella group' + Megalosphyinae).
菌蚊科是一个物种多样性很大的科，分布于世界各地，包括重要的农业害虫——栽培蘑菇和温室植物。本研究测定了 5 个代表菌蚊科 3 个亚科的近乎完整的线粒体基因组。这些线粒体基因组的长度范围从 13849bp 到 16923bp，包含 13 个蛋白质编码基因（PCGs）、20-22 个转移 RNA（tRNA）基因、2 个核糖体 RNA（rRNA）基因和一个控制区（CR）。与其他双翅目物种相比，菌蚊科的重排更为常见。trnL2 经常发生倒位或转换，在 tRNA 簇 trnI-trnQ-trnM、trnW-trnC-trnY 和 trnA-trnR-trnN-trnS1-trnE-trnF 中也是如此。本研究基于这些新测序的物种以及已发表的相关科的线粒体基因组，重建了科内的系统发育关系，恢复了 Sciaroidea 作为 Cecidomyiidae + (Sciaridae + Keroplatidae) 的拓扑结构。在菌蚊科内恢复的关系是 Sciarinae + ('Pseudolycoriella 组' + Megalosphyinae)。
