Sate Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Metabolic Innovation Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 540080, China.
J Hazard Mater. 2020 May 5;389:122109. doi: 10.1016/j.jhazmat.2020.122109. Epub 2020 Jan 21.
Perfluorooctanoic acid (PFOA), as a typical emerging organic pollutant, can interact with serum albumin. However, it remains to characterize the binding of PFOA with serum albumin and to address the role of this interaction in related toxic effects. We aimed to characterize the interaction between PFOA and albumin for understanding the effects of this interaction on the uptake, distribution, and toxicity of PFOA in human cells. The results showed that viable cell count was significantly enhanced by addition of fetabl bovine serum (FBS) into cell culture medium with 300 μM PFOA treatment. PFOA mainly existed as complexed with FBS, at FBS concentration > 10%, which substantially reduced the absorption efficiency of all cell lines to PFOA. The majority of PFOA was accumulated in the cytosolic fraction, followed by nuclei, and mitochondria. Conclusively, our study suggests that the complexation of organic contaminants with proteins might mitigate their toxicity by reducing cellular uptake.
全氟辛酸（PFOA）作为一种典型的新兴有机污染物，可以与血清白蛋白相互作用。然而，目前仍需对 PFOA 与血清白蛋白的结合进行特征描述，并阐明这种相互作用在相关毒性效应中的作用。我们旨在对 PFOA 与白蛋白的相互作用进行特征描述，以了解这种相互作用对 PFOA 在人细胞中摄取、分布和毒性的影响。结果表明，在含有 300 μM PFOA 的细胞培养液中加入胎牛血清（FBS），可显著提高活细胞计数。当 FBS 浓度＞10%时，PFOA 主要以与 FBS 结合的形式存在，这大大降低了所有细胞系对 PFOA 的吸收效率。大多数 PFOA 积聚在细胞质部分，其次是细胞核和线粒体。总之，我们的研究表明，通过减少细胞摄取，有机污染物与蛋白质的复合可能会降低其毒性。
