Department of Pediatrics, Cardiovascular Research, Masonic Cancer Center, Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA.
Methods Mol Biol. 2020;2115:435-444. doi: 10.1007/978-1-0716-0290-4_24.
The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.
CRISPR/Cas9 系统可实现人类原代细胞的基因定点编辑和基因组工程。本文描述了使用 CRISPR/Cas9 对从人外周血单核细胞中分离的 B 细胞进行基因编辑和基因组工程的方法。使用这种方法可以达到高达 90%的编辑频率和超过 60%的整合率。
