Department of Medical Microbiology, Hamidiye Faculty of Medicine, University of Health Sciences, İstanbul, Turkey.
Department of Parasitology, Faculty of Medicine, Ege University, İzmir, Turkey.
Acta Trop. 2020 Apr;204:105362. doi: 10.1016/j.actatropica.2020.105362. Epub 2020 Jan 29.
Head lice infestation caused by Pediculus humanus capitis De Geer, 1767 is one of the most common public health problems. The relationship between humans and head lice dates back millions of years ago that differentiated into different phylogenetic clades. Treatment of head lice infestation usually based on insecticide-based products, which promotes the resistance in the head lice populations. In the present study, we aimed to screen the presence of permethrin resistance among collected P. h. capitis specimens in Turkey. Three mutation sites (T917I, L920F, and M815I) were screened using real-time PCR and resistance was identified by melt analysis. Of the studied specimens, resistance allele frequency (RAF) was found 0.98 for T917I, 0.99 for L920F, and 1.00 for M815I. The phylogenetic study revealed that Clade A and Clade B are present and overlap in Turkey. The present study is first to screen the resistance among Turkish head lice specimens. To not stimulate the pyrethroids resistance in head lice populations, early detection of resistance is crucial and will help the health professionals to choose suitable formula in the treatment. We suggest that the resistance status needs to be screened in randomly selected populations before any treatment application is given.
人头虱寄生引起的头虱感染是最常见的公共卫生问题之一。人类与头虱的关系可以追溯到数百万年前，那时它们已经分化为不同的进化枝。头虱感染的治疗通常基于杀虫剂产品，这促进了头虱种群的抗药性。在本研究中，我们旨在筛选土耳其采集的人头虱标本中是否存在氯菊酯抗性。我们使用实时 PCR 筛选了三个突变位点（T917I、L920F 和 M815I），并通过熔解分析鉴定了抗性。在所研究的标本中，T917I 的抗性等位基因频率（RAF）为 0.98，L920F 为 0.99，M815I 为 1.00。系统发育研究表明，土耳其存在 A 群和 B 群，并存在重叠。本研究首次筛选了土耳其人头虱标本中的抗性。为了不刺激头虱种群对拟除虫菊酯的抗性，早期检测抗性至关重要，这将有助于卫生专业人员在治疗中选择合适的配方。我们建议在进行任何治疗应用之前，需要在随机选择的人群中筛查抗性状况。
