Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Institute of Chemical Biology and Fundamental Medicine, Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia.
Infect Genet Evol. 2020 Jun;80:104214. doi: 10.1016/j.meegid.2020.104214. Epub 2020 Jan 30.
Parechovirus (the Picornaviridae family), recently classified as Parechovirus A and formerly known as Human parechovirus (HPeV), can cause a wide range of human diseases. Over the past decade, several studies have reported HPeV epidemiology in different regions; however, information from Russia is limited. A total of 632 stool samples collected in Novosibirsk, Russia during January-March 2012 were screened for HPeV by RT-PCR. The study cohort comprised 572 patients with acute gastroenteritis and 60 healthy children. Seven of 572 (1.2%) gastroenteritis cases were HPeV-positive, including one co-infection with rotavirus and astrovirus. All positive patients were ≤1 year old, and five of them were younger than 3 months. None of the healthy controls provided an HPeV-positive sample. Six HPeV isolates were classified as HPeV-1 and one as HPeV-5 using phylogenetic analysis. Two complete genome sequences of HPeV-1 and one of HPeV-5 were determined and analyzed. Phylogenetic analysis showed that the studied Russian strains are probably recombinants. P1 region sequences of two Russian HPeV-1 strains clustered with rare contemporary HPeV-1A strains, whereas their P3 regions were phylogenetically closer to the archival Harris strain. The Russian HPeV-5 strain formed a common cluster with other HPeV-5 strains only for the P1 region, while the P3 region grouped with the German HPeV-2 strain. In the Russian HPeV-5 strain, the lack of the arginine-glycine-aspartic acid (RGD) motif at the C-terminus of VP1 was observed. This is the first complete genome characterization of the Russian HPeV strains detected in sporadic cases of pediatric acute gastroenteritis.
肠道病毒（小核糖核酸病毒科），最近被分类为肠道病毒 A，以前称为人类肠道病毒（HPeV），可引起广泛的人类疾病。在过去的十年中，已有多项研究报告了不同地区的 HPeV 流行病学情况；然而，来自俄罗斯的信息有限。2012 年 1 月至 3 月，俄罗斯新西伯利亚共采集了 632 份粪便样本，通过 RT-PCR 筛查 HPeV。研究队列包括 572 例急性胃肠炎患者和 60 例健康儿童。572 例胃肠炎病例中有 7 例（1.2%）为 HPeV 阳性，其中 1 例合并轮状病毒和星状病毒感染。所有阳性患者均≤1 岁，其中 5 例小于 3 个月。健康对照组均未提供 HPeV 阳性样本。使用系统进化分析将 6 株 HPeV 分离株归类为 HPeV-1，1 株归类为 HPeV-5。测定并分析了 HPeV-1 的两个完整基因组序列和 HPeV-5 的一个完整基因组序列。系统进化分析表明，所研究的俄罗斯株可能是重组株。两种俄罗斯 HPeV-1 株的 P1 区序列与罕见的当代 HPeV-1A 株聚类，而其 P3 区与档案哈里斯株亲缘关系更近。俄罗斯 HPeV-5 株仅在 P1 区与其他 HPeV-5 株形成共同聚类，而 P3 区与德国 HPeV-2 株聚类。在俄罗斯 HPeV-5 株中，VP1 羧基末端缺乏精氨酸-甘氨酸-天冬氨酸（RGD）基序。这是首次对俄罗斯 HPeV 株进行的散发性儿科急性胃肠炎病例的完整基因组特征描述。
